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ID   PCYM00     preliminary; circular DNA; SYN; 9408 BP.
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AC   ATCC37821;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/vertebrate/E.coli plasmid vector pCYM00 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCYM0 from pSV2-cat & pUT332
RC   pCYM1 from pCYM0
RC   pCYM2 from pCYM0 & YRp7
RC   pCYM3 from pCYM2 & pTG2856
RC   pCYM00 from pCYM0 & pMC1403
RC   pCYM01 from pCYM1 & pMC1403
RC   pCYM02 from pCYM2 & pMC1403
RC   pCYM03 from pCYM3 & pMC1403
RC   pCYM1-11 from pCYM1 & M13mp11
RC   pCYM3-11 from pCYM3 & M13mp11
RA   Camonis J.H., Cassan M., Rousset J.P.;
RT   "Of mice and yeast: versatile vectors which permit gene expression
RT   in both budding yeast and higher eukaryotic cells";
RL   Gene 86:263-268(1990).
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CC   Constructed by inserting a BamHI/SalI fragment from pMC1403 (lac
CC   operon beginning at codon 9 of lacZ) between the BamHI/BalI
CC   (SalI-linkered) sites of pCYM0.
CC   Expresses beta-galactosidase activity in E.coli, S. cerevisiae, and
CC   mouse cells, and thus can be used as a control to calibrate
CC   transformation efficiency.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI/SalI--6.0(doublet); EcoRI--12.0; SmaI--12.0; Pvu/BamHI--6.5,
CC   2.5, 2.1, 0.54, 0.49. (ATCC staff)
CC   Our plasmid is 12 kb instead of 9.4, which matches the original
CC   depositor's DNA. (ATCC staff)
CC   Shuttle reporter plasmid.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCYM00)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli CM5alpha)(Saccharomyces cerevisiae)(vertebrate cells)
CC   HO (E.coli)(Saccharomyces cerevisiae W303-1B/D)(mouse C11D cell line)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pCYM0 remove BamHI-BalI
FT                   \ pSV2-cat BamHI-BalI 1119bp 3370..4489
FT                   \ yeast 2 micron BalI 267
FT                   :SalI linker 6bp gtcgac
FT                   2. pMC1403 BamHI-?SalI 3650..?, lacZ gene
FT                   -> pCYM00 9408bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SmaI-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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