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ID   PCYM01     preliminary; circular DNA; SYN; 8745 BP.
AC   ATCC37823;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/vertebrate/E.coli plasmid vector pCYM01 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pCYM0 from pSV2-cat & pUT332
RC   pCYM1 from pCYM0
RC   pCYM2 from pCYM0 & YRp7
RC   pCYM3 from pCYM2 & pTG2856
RC   pCYM00 from pCYM0 & pMC1403
RC   pCYM01 from pCYM1 & pMC1403
RC   pCYM02 from pCYM2 & pMC1403
RC   pCYM03 from pCYM3 & pMC1403
RC   pCYM1-11 from pCYM1 & M13mp11
RC   pCYM3-11 from pCYM3 & M13mp11
RA   Camonis J.H., Cassan M., Rousset J.P.;
RT   "Of mice and yeast: versatile vectors which permit gene expression
RT   in both budding yeast and higher eukaryotic cells";
RL   Gene 86:263-268(1990).
CC   The EcoRI, SmaI, and BamHI sites are 3' to the unique available
CC   in-phase initiation codon under control of the SV40 early promoter.
CC   Expresses beta-galactosidase activity in E.coli, S. cerevisiae, and
CC   mouse cells, and thus can be used as a control to calibrate
CC   transformation efficency.
CC   Constructed by inserting a 3.048 kb EcoRI/DraI fragment from pMC1403
CC   into pCYM1 cut with BamHI (nt 427), linkered to add an EcoRI site, and
CC   cut with BalI (nt 702).
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI/PvuII--4.8, 2.6, 0.5, 0.46; EcoRI--8.4; SmaI--8.4. (ATCC staff)
CC   Also permits blue/white detection in E.coli.
CC   Shuttle expression vector which includes a reporter group. (ATCC
CC   staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCYM01)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli CM5alpha)(Saccharomyces cerevisiae)(vertebrate cells)
CC   HO (E.coli)(Saccharomyces cerevisiae W303-1B/D)(mouse C11D cell line)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pCYM1 BamHI 5697bp
FT                   \ pSV2-cat BamHI 3370..3370
FT                   mung bean nuclease
FT                   EcoRI linker 12bp nnngaattcnnn
FT                   BalI 5619bp,
FT                   \ yeast 2 micron 267..267/pSV2-cat 4489..4489
FT                   2. pMC1403 EcoRI-DraI 3126bp 3640..6766, lacZ gene
FT                   \ [3048bp]
FT                   \ DraI = 2513 2532 3224 6766 7757 8682
FT                   -> pCYM01 8745bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SmaI-BamHI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast URA3 gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;