back Return to this vector's summary.
ID   PCYM2      preliminary; circular DNA; SYN; 5462 BP.
XX
AC   ATCC37824;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Saccharomyces/vertebrate/E.coli plasmid vector pCYM2 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCYM0 from pSV2-cat & pUT332
RC   pCYM1 from pCYM0
RC   pCYM2 from pCYM0 & YRp7
RC   pCYM3 from pCYM2 & pTG2856
RC   pCYM00 from pCYM0 & pMC1403
RC   pCYM01 from pCYM1 & pMC1403
RC   pCYM02 from pCYM2 & pMC1403
RC   pCYM03 from pCYM3 & pMC1403
RC   pCYM1-11 from pCYM1 & M13mp11
RC   pCYM3-11 from pCYM3 & M13mp11
RA   Camonis J.H., Cassan M., Rousset J.P.;
RT   "Of mice and yeast: versatile vectors which permit gene expression
RT   in both budding yeast and higher eukaryotic cells";
RL   Gene 86:263-268(1990).
XX
CC   The EcoRI, SmaI, and BamHI sites are 3' to the unique available
CC   in-phase initiation codon under control of the SV40 early promoter.
CC   Constructed by deleting the HpaI (nt 1686)/BglII (nt 3463) site from
CC   pCYM0 and inserting the 879 bp BglII/EcoRI fragment containing TRP1
CC   from YRp7.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--5.2; BglII/EcoRI--3.2, 2.0; EcoRI--5.2; BamHI/PvuII--4.5, 0.51.
CC   (ATCC staff)
CC   Shuttle expression vector. (ATCC staff)
CC   Permits expression in both S. cerevisiae and mouse cells. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCYM2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli CM5alpha)(Saccharomyces cerevisiae)(vertebrate cells)
CC   HO (E.coli)(Saccharomyces cerevisiae W303-1B/D)(mouse C11D cell line)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pCYM0 remove HpaI-BglII 1500bp,
FT                   \ BglII in URA3 gene/4500bp
FT                   \ yeast 2 micron HpaI = 2967
FT                   \ pSV2-cat HpaI = 3505
FT                   fill in:
FT                   2. YRp7 BglII-EcoRI 852bp 604..1456, TRP1 gene [879bp]
FT                   fill in:
FT                   -> pCYM2 5462bp [no HpaI]"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-SmaI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-SmaI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//