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ID   PCYM3      preliminary; circular DNA; SYN; 6543 BP.
AC   ATCC37826;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/vertebrate/E.coli plasmid vector pCYM3 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pCYM0 from pSV2-cat & pUT332
RC   pCYM1 from pCYM0
RC   pCYM2 from pCYM0 & YRp7
RC   pCYM3 from pCYM2 & pTG2856
RC   pCYM00 from pCYM0 & pMC1403
RC   pCYM01 from pCYM1 & pMC1403
RC   pCYM02 from pCYM2 & pMC1403
RC   pCYM03 from pCYM3 & pMC1403
RC   pCYM1-11 from pCYM1 & M13mp11
RC   pCYM3-11 from pCYM3 & M13mp11
RA   Camonis J.H., Cassan M., Rousset J.P.;
RT   "Of mice and yeast: versatile vectors which permit gene expression
RT   in both budding yeast and higher eukaryotic cells";
RL   Gene 86:263-268(1990).
CC   The EcoRI, SmaI, and BamHI sites are 3' to the unique available
CC   in-phase initiation codon under control of the SV40 early promoter.
CC   Shuttle expression vector. (ATCC staff)
CC   Permits expression in both S. cerevisiae and mouse cells. (ATCC staff)
CC   Constructed by inserting a 1.207 kb ClaI/BamHI fragment containing
CC   URA3d into pCYM2 cut with BglII & ClaI (nt 3469 and 3589 according to
CC   the pCYM0 numbering scheme).
CC   URA3d is a modified URA3 gene which is not efficiently expressed and
CC   requires a large number of plasmids to complement a ura3 mutation.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BglII--uncut; ClaI/BamHI--3.4,2.8,0.5; EcoRI--6.6; BamHI/PvuII--6.0,
CC   0.5. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCYM3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli CM5alpha)(Saccharomyces cerevisiae)(vertebrate cells)
CC   HO (E.coli)(Saccharomyces cerevisiae W303-1B/D)(mouse C11D cell line)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pCYM2 remove BglII-ClaI 120bp,
FT                   \ pCYM0 3469..3589/5336bp [5342bp]
FT                   2. pTG2856 BamHI-ClaI 1207bp, URA3d gene
FT                   \ pBR322 ClaI-BamHI 25..376/yeast 2 micron ClaI 6286
FT                   -> pCYM3 6543bp"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-SmaI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-SmaI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3d gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;