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ID   PCYM311    preliminary; circular DNA; SYN; 6485 BP.
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AC   ATCC37829;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/vertebrate/E.coli plasmid vector pCYM3-11 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pCYM0 from pSV2-cat & pUT332
RC   pCYM1 from pCYM0
RC   pCYM2 from pCYM0 & YRp7
RC   pCYM3 from pCYM2 & pTG2856
RC   pCYM00 from pCYM0 & pMC1403
RC   pCYM01 from pCYM1 & pMC1403
RC   pCYM02 from pCYM2 & pMC1403
RC   pCYM03 from pCYM3 & pMC1403
RC   pCYM1-11 from pCYM1 & M13mp11
RC   pCYM3-11 from pCYM3 & M13mp11
RA   Camonis J.H., Cassan M., Rousset J.P.;
RT   "Of mice and yeast: versatile vectors which permit gene expression
RT   in both budding yeast and higher eukaryotic cells";
RL   Gene 86:263-268(1990).
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CC   The EcoRI, SmaI, and BamHI sites are 3' to the unique available
CC   in-phase initiation codon under control of the SV40 early promoter.
CC   Constructed by replacing the HindIII/EcoRI fragment of pCYM3 with the
CC   HindIII/EcoRI multiple cloning site from M13mp11.  The translation
CC   start codon has been removed by this process.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--6.3; ClaI/BamHI--3.3, 2.6, 0.5; HindIII/EcoRI--3.3, 2.0, 1.2.
CC   (ATCC staff)
CC   Shuttle expression vector. (ATCC staff)
CC   Permits expression in both S. cerevisiae and mouse cells. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pCYM3-11)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli CM5alpha)(Saccharomyces cerevisiae)(vertebrate cells)
CC   HO (E.coli)(Saccharomyces cerevisiae W303-1B/D)(mouse C11D cell line)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pCYM3 remove small HindIII-EcoRI, 6543bp-
FT                   2. M13mp11 HindIII-EcoRI 45bp 6235..6280, MCS
FT                   -> pCYM3-11 6485bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SmaI-BamHI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast TRP1 gene"
FT   CDS             0..0
FT                   /note="ANT yeast URA3d gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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