back Return to this vector's summary.
ID   PCYTEXP1   preliminary; circular DNA; SYN; 179 BP.
XX
AC   IG5178;
XX
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pCYTEXP1 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pCYTEXP1 from linker & pUC-f1 & pJLA603
RA   Belev T.N., Singh M., McCarthy J.E.;
RT   "A fully modular vector system for the optimization of gene
RT   expression in Escherichia coli";
RL   Plasmid 26:147-150(1991).
XX
CC   MCS. fd terminator. oligonucleotide linker.
CC   NM (pCYTEXP1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pJLA603)(pUC-f1)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. phage fd, terminator
FT                   2. lambda, cIts857 gene/pR/pL
FT                   -> pJLf201
FT                   1. pJLf201
FT                   2. E. coli, atpETIR gene
FT                   -> pJLA503
FT                   1. pJLA503
FT                   2. oligo
FT                   -> pJLA603
FT                   1. pJLA603 PstI, 5' to cIts857 gene
FT                   NotI-PstI linker 14bp gcggccgcctgcag
FT                   -> plasmid
FT                   1. plasmid remove SalI-NarI, MCS to phage fd term
FT                   2. oligo SalI-XbaI-NarI
FT                   -> plasmid2
FT                   1. plasmid2 XbaI, MCS
FT                   2. pJLA603, phage fd terminator
FT                   XbaI linker 6bp tctaga:XbaI linker 6bp tctaga
FT                   -> plasmid3
FT                   1. plasmid3 NotI
FT                   2. pUC-f1 EcoRI-BamHI 535bp 397..932, f1 ori
FT                   mung bean nuclease
FT                   -> pCYTEXP1 [no PstI 5' to cIts857 gene]"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 179 BP; 53 A; 41 C; 44 G; 41 T; 0 other;
     tgtgtgatac gaaacgaagc attggcgcct cgagtaattt accaacacta ctacgtttta
     actgaaacaa actggagact catatggcat gcacaggaac cggatcctaa gtaagtagaa
     ttctgagtag gtaagtcgac aatcgcgtct agagcccgac gcgctgggct acgtcttgc
//