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ID   PDA71      preliminary; DNA; SYN; 8800 BP.
AC   ATCC77474;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Rhodococcus/E.coli plasmid vector pDA71 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pDA71 from pBR322 & Rhodococcus rhodochrous cat gene
RA   Quan S., Dabbs E.R.;
RT   "Nocardioform arsenic resistance plasmid characterization and
RT   improved Rhodococcus cloning vectors";
RL   Plasmid 29:74-79(1993).
CC   Shuttle vector permitting positive selection in E. coli. Cloning
CC   sites are positioned in the EcoRI endonuclease gene, regulated by a
CC   lambda promoter. In E. coli hosts providing the temperature-sensitive
CC   lambda repressor cI857, growth at 37C will occur only if the
CC   endonuclease gene is interrupted. The non-enzymatic chloramphenicol
CC   resistance gene, derived from Rhodococcus rhodochrous SC2318 (ATCC
CC   12674), does not confer resistance in E. coli. The plasmid confers
CC   immunity to nocardiophage Q4. [1]
CC   Growth media: LB plus ampicillin (ATCC medium number 1227) 30C
CC   Deposited by: E.R.Dabbs
CC   NM (pDA71)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (Rhodococcus)(E.coli)
CC   CP ()
CC   FN (cloning)(shuttle)(library)(hsdR restriction endonuclease EcoRI)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. Rhodococcus rhodochrous, cat gene
FT                   3. lambda, promoter
FT                   -> pDA71 8800bp"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT E. coli cat gene"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT SfuI"
FT   promoter        0..0
FT                   /note="PRO lambda"
FT   CDS             0..0
FT                   /note="GEN Rhodococcus hsdR gene"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI Rhodococcus phage Q4"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;