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ID   PDAH274    preliminary; circular DNA; SYN; 91 BP.
AC   IG5102;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pDAH274 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pTUC1 from pUC105 & pTEBG3
RC   pDAH210 from pIC19H & pTEBG3
RC   pDAH211 from pMC871 & pREG422
RC   pDAH216 from pMC871 & pDAH210
RC   pDAH274 from pDAH216 & pDAH211
RC   pLACPB1, pLACPB2 from pUC105 & pDAH216 & pDAH274
RA   Scanlan D.J., Bloye S.A., Mann N.H., Hodgson D.A., Carr N.G.;
RT   "Construction of lacZ promoter probe vectors for use in
RT   Synechococcus: application to the identification of CO2-regulated
RT   promoters";
RL   Gene 90:43-49(1990).
RN   [2]
RC   pREG411 from pBR322 & phage P1
RC   pLJS9 from pREG411 & Tn5 omega1519
RC   pLJS10 from pREG411 & Tn5 omega1519
RC   pLJS11 from pREG411 & Tn5 omega1519
RC   pLJS12 from pREG411 & Tn5 omega1519
RC   [pREG153 from pBR322 & phage P1]
RC   [pREG422 from pBR322 & phage P1]
RA   Shimkets L.J., Gill R.E., Kaiser D.;
RT   "Developmental cell interactions in Myxococcus xanthus and the spoC
RT   locus";
RL   Proc. Natl. Acad. Sci. U.S.A. 80:1406-1410(1983).
RN   [3]
RC   pTEBG3 from pBR322 & lacZ gene
RA   Elledge S.;
RT   ;
RL   Unpublished (1990).
CC   not original sequence. fusion region.
CC   NM (pDAH274)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pIC19H)(pTEBG3)(pMC871)(pLACPB1 & pLACPB2 from pUC105)
CC   BR (pDAH216)
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 HindIII 4361bp 30..30
FT                   2. E. coli EcoRI, lacZ gene
FT                   mutagenesis [no EcoRI]
FT                   HindIII-HindIII, lacZ gene
FT                   -> pTEBG3
FT                   1. pIC19H remove ClaI-BglII 9bp 271..280, MCS/2692bp
FT                   2. pTEBG3 BamHI-ClaI 2600bp, 3' lacZ gene
FT                   \ pBR322 25..-..376
FT                   -> pDAH210 5300bp
FT                   1. pBR322 EcoRI 4361bp 4360..4360
FT                   2. phage P1 EcoRI-EcoRI 6700bp, ori/kan gene
FT                   -> pREG422 11000bp [pMB1 ori/amp gene/P1 ori/kan gene]
FT                   1. ColE1
FT                   2. E. coli, lacZ
FT                   -> pMC81
FT                   1. pMC81
FT                   2. pACYC177
FT                   -> pMC275
FT                   1. pMC275
FT                   -> pMC874
FT                   1. pBR322
FT                   2. E. coli, lacZ
FT                   -> pMC489
FT                   1. pMB9
FT                   2. pMC489
FT                   -> pMC511
FT                   1. pMC874
FT                   2. pMC511
FT                   -> pMC871
FT                   1. pMC871
FT                   2. pREG422
FT                   -> pDAH211
FT                   1. pMC871 small TaqI-TaqI, 5' lacZ gene
FT                   2. pDAH210 ClaI 5300bp, pIC19H 271..271
FT                   -> pDAH216
FT                   1. pDAH216 HindIII-HindIII
FT                   fill in:fill in
FT                   2. pDAH211 EcoRI-BamHI
FT                   fill in:fill in
FT                   -> pDAH274"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 91 BP; 22 A; 27 C; 20 G; 22 T; 0 other;
     gcggcgacgc gcagttaatc ccacagccgc cagttccgct ggcggcattt taactttctt
     tatcacacag gaaacagcta tgaccatgat t