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ID   PDB248     preliminary; circular DNA; SYN; 10100 BP.
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AC   IG7010;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Schizosaccharomyces plasmid vector pDB248 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pDB248 from pJDB248 & pBR322
RC   pDamP2, pDamP series from pDAM1 & S. pombe ARS
RC   [pDB262 from pBR322 & LEU2 gene & lambda, cI gene/pR]
RC   [pWH4 from pDB262]
RC   [pWH5 from pWH4]
RA   Beach D., Nurse P.;
RT   "High-frequency transformation of the fission yeast
RT   Schizosaccharomyces pombe";
RL   Nature 290:140-142(1981).
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RN   [2]
RC   pDAM1 from pBR325 & pJDB248
RC   pDAMY1 from pDAM1 & yeast ARS
RC   pDAMY2 from pDAM1 & yeast ARS
RC   pDAMY3 from pDAM1 & yeast ARS
RC   pDAMY4 from pDAM1 & yeast ARS
RC   pDAMY5 from pDAM1 & yeast ARS
RC   pDAMY6 from pDAM1 & yeast ARS
RC   pDAMY7 from pDAM1 & yeast ARS
RC   pDAMY8 from pDAM1 & yeast ARS
RA   Beach D.H., Piper M., Shall S.;
RT   "Isolation of chromosomal origins of replication in yeast";
RL   Nature 284:185-187(1980).
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CC   NM (pDB248)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (Saccharomyces)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (2 micron plasmid)
CC   BR ()
CC   OF (pSMF32T)((pSMF35T)(pSMF38T)
CC   OR (yes)
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pMB9 EcoRI-EcoRI 1233bp, ori
FT                   2. yeast EcoRI-EcoRI 4400bp, LEU2 gene
FT                   3. yeast 2 micron B form EcoRI-EcoRI 2235bp
FT                   \ 2408..3942 & 703..2
FT                   \ UL = 3239bp 703..3942 US = 3079bp 3942..6318..703 A
FT                   \ UL = 3239bp 703..3942 US = 3079bp 703..6318..3942 B
FT                   -> pJDB248 7900bp
FT                   1. pJDB248 remove EcoRI-EcoRI 2235bp, yeast 2 micron
FT                   \ 2408..3942 & 703..2 & EcoRI-EcoRI 4400bp LEU2/5700bp
FT                   2. pBR322 EcoRI 4361bp 4360..4360, amp/tet/ori
FT                   -> pDB248 10100bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron ori"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique HpaI"
FT   misc_feature    0..0
FT                   /note="PstI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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