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ID   PDBPVMMTNE preliminary; circular DNA; SYN; 14600 BP.
AC   ATCC37224;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pdBPV-MMTneo(342-12) - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pML2d from pBR322
RC   G418-cassette from mouse MT I prom & Tn5 neo & SV40 early prom
RC   pdBPV-MMTneo(342-12) from BPV-1 & pML2d & G418-cassette
RC   pdMMTneo(302-3) from pML2d & G418-cassette
RA   Law M.F., Byrne J.C., Howley P.M.;
RT   "A stable bovine papillomavirus hybrid plasmid that expresses a
RT   dominant selective trait";
RL   Mol. Cell. Biol. 3:2110-2115(1983).
CC   A stable extrachromosomal plasmid which can be positively selected for
CC   in mouse cells.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pdBPV-MMTneo 342-12)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI-HindIII 4015bp 376..4361..30
FT                   2. BPV-1 HindIII-BamHI 5480bp, BPV-1 69% fragment
FT                   \ BPV-1 = 7945bp
FT                   -> pBPV69T(43-1) 9500bp
FT                   1. pML2 BamHI 2900bp 306..306
FT                   2. pBPV69T(43-1) HindIII-BamHI 5480bp, BPV-1 69% frag
FT                   \ BPV-1 = 7945bp
FT                   -> pd1BPV69T(51-1) 8400bp
FT                   1. pd1BPV69T(51-1) HindIII 8400bp, BPV-1
FT                   BamHI linker 6bp ggatcc
FT                   -> pd1BPV69T(54-2) 8400bp
FT                   1. pd1BPV69T(54-2) remove BamHI-BamHI 5800bp, 2600bp
FT                   -> pML2d 2600bp [unique BamHI]
FT                   1. mouse EcoRI-BglII, MT I promoter
FT                   2. Tn5 SalI 5818bp 2685..2685, neo/kan gene
FT                   Klenow
FT                   BglII-XhoI 1317bp 1516..2833, neo/kan gene
FT                   3. SV40 MboI-StuI 835bp 4771..5243..363,
FT                   \ early promoter/splice
FT                   -> G418-cassette
FT                   1. BPV-1 BamHI 7945bp
FT                   2. pML2d BamHI-HindIII, [inverted dup]
FT                   2. pML2d HindIII-EcoRI, [inverted dup]
FT                   3. G418-cassette EcoRI-BglII, MT I promoter
FT                   -> pdBPV-MMTneo(342-12) 14600bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BglII-EcoRI-SalI-HpaI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO mouse MMT gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT mouse G418 resistance gene (G418)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;