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ID   PDC47      preliminary; circular DNA; SYN; 6100 BP.
AC   ATCC87028;
DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Saccharomyces/vertebrate/E.coli plasmid pDC47 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   HIS3/pSV2neo from pSV2neo & HIS3 gene
RC   BLUR13.RI from BLUR13 & linker
RC   pBP47 from HIS3/pSV2neo & BLUR13.RI
RC   [pPOL2shortneobpA from pBR322 & neo gene]
RC   [pDC47 from pPOL2shortneobpA & pBP47]
RA   Pavan W.J., Hieter P., Reeves R.H.;
RT   "Modification and transfer into an embryonal carcinoma cell line
RT   of a 360-kilobase human-derived yeast artificial chromosome";
RL   Mol. Cell. Biol. 10:4163-4169(1990).
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--5.8, 0.3; PvuI--6.1; EcoRI--4.0, 2.3. (ATCC staff)
CC   YI-type shuttle integrative vector useful for incorporating a neoR
CC   cassette into YACs. Constructed by ligating the 1.35 kb XhoI fragment
CC   of pPOL2shortneobpA to the largest PvuII fragment of pBP47.
CC   Linearizing with SmaI and transformating into a YAC-containing his3
CC   deletion strain will target integration to Alu sequences.
CC   Linearizating at the PvuI site will target integration to the TRP1
CC   centric arm of pYAC4 derivatives. His+ transformants can then be
CC   transferred to a eukaryotic cell by spheroplast fusion.
CC   The order of the major features in this plasmid is:  pMB1 ori - bla -
CC   HIS3 - BamHI - SmaI - BamHI - pol2sneo. (personal communication)
CC   Growth: LB plus ampicillin (ATCC medium number 1227) 37C
CC   Deposited by: Reeves R.H.
CC   NM (pDC47)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)(E.coli DH5alpha)(Saccharomyces cerevisiae)
CC   HO (vertebrate cells)
CC   CP ()
CC   FN (cloning)(shuttle - integrating)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. Tn5, neo gene
FT                   -> pPOL2shortneobpA
FT                   1. pPOL2shortneobpA
FT                   2. pBP47
FT                   -> pDC47 6100bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   CDS             0..0
FT                   /note="ANT yeast HIS3 gene"
FT   CDS             0..0
FT                   /note="ANT vertebrate G418 resistance gene"
FT   terminator      0..0
FT                   /note="TER SV40"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;