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ID   PDDGAL15   preliminary; circular DNA; SYN; 8500 BP.
AC   ATCC37770;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Dictyostelium/E.coli plasmid vector pDdGal-15 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pDdGal-15 from pB10TP2 & pDdlac-1
RC   pDdGal-16 from pB10TP2 & pDdlac-1
RC   pDdGal-17 from pB10TP2 & pDdlac-1
RA   Harwood A.J., Drury L.A.;
RT   "New vectors for expression of the E.coli lacZ gene in
RT   Dictyostelium";
RL   Nucleic Acids Res. 18:4292-4292(1990).
RN   [2]
RC   pDdlac-1 from lacZ gene & Dictyostelium actin 8 terminator
RA   Dingermann T., Reindl N., Werner H., Hildebrandt M., Nellen W.,
RA   Harwood A.J., Williams J.G., Nerke K.;
RT   "Optimization and in situ detection of Escherichia coli
RT   beta-galactosidase gene expression in Dictyostelium discoideum";
RL   Gene 85:353-362(1989).
RN   [3]
RC   pDdlac-1 from lacZ gene & Dictyostelium actin 8 terminator
RA   Williams J.G., Duffy K.T., Lane D.P., McRobbie S.J., Harwood A.J.,
RA   Traynor D., Kay R.R., Jermyn K.A.;
RT   "Origins of the prestalk-prespore pattern in Dictyostelium
RT   development";
RL   Cell 59:1157-1163(1989).
CC   pDdGal-15 (ATCC 37770), pDdGal-16 (ATCC 37771) and pDdGal-17 (ATCC
CC   37772) differ in their polylinker sequences.  Each contains a BglII
CC   site in a different reading frame relative to the lacZ gene.
CC   A promoter-cloning plasmid vector using expression of
CC   beta-galactosidase as the reporter in Dictyostelium.
CC   The lacZ gene and Dictyostelium actin 8 terminator from pDdlac-1 were
CC   inserted into a derivative of pB10TP2 from which the f1 origin and and
CC   a lacZ fragment had been removed.
CC   The neo gene is regulated by an actin 6 promoter and a CP1 terminator.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--8.5; BamHI--8.5; BglII--8.5. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pDdGal-15)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(Dictyostelium discoideum)(E.coli SURE)(E.coli DH5)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pDdlac-1)(pB10TP2 from pEMBL18+)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. E. coli, lacZ gene
FT                   2. Dictyostelium actin 8 terminator
FT                   -> pDdlac-1
FT                   1. pB10TP2 remove XbaI-HindIII, f1 ori/lacZ
FT                   2. pDdlac-1 XbaI-HindIII, MCS/lacZ gene/actin 8 term
FT                   -> pDdGal-15 8500bp"
FT   misc_binding    0..0
FT                   /note="MCS XbaI-HindIII-EcoRI-BglII-PstI-BamHI-SmaI"
FT   misc_binding    0..0
FT                   /note="SIT unique XbaI-EcoRI-BglII-BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT Dictyostelium G418 resistance gene (G418)"
SQ   Sequence 54 BP; 20 A; 10 C; 11 G; 13 T; 0 other;
     tctagaaagc ttaaaaaaat gaattctaag atctctgcag ggatcccggg caat