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ID   PDELTA2    preliminary; circular DNA; SYN; 7960 BP.
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AC   IG9012;
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DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pDELTA2 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [pDELTA2 from pUC19 & gamma-delta]
RA   Wang G., Berg C.M., ChenJ., Young A.C., Blakesley R.W., Lee L.Y.,
RA   Berg D.E.;
RT   ;
RL   Focus 15:47-47(1993).
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CC   NM (pDELTA2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (BRL)
CC   HO (E.coli)
CC   CP (high)
CC   FN (cloning)(expression)(transcription)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp
FT                   2. gamma-delta
FT                   -> pDELTA2 7960bp"
FT   misc_binding    0..0
FT                   /note="SIT KpnI"
FT   misc_binding    0..0
FT                   /note="SIT BssHII"
FT   promoter        0..0
FT                   /note="PRO bacteriophage Sp6"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   CDS             complement(0..0)
FT                   /note="GEN sacB gene"
FT   misc_binding    0..0
FT                   /note="SIT SnaBI"
FT   misc_binding    0..0
FT                   /note="SIT Bsp1407I"
FT   misc_binding    0..0
FT                   /note="SIT BsgI"
FT   misc_binding    0..0
FT                   /note="SIT MscI"
FT   misc_binding    0..0
FT                   /note="SIT StuI"
FT   CDS             complement(0..0)
FT                   /note="ANT kan gene"
FT   misc_binding    0..0
FT                   /note="SIT PfiMI"
FT   misc_binding    0..0
FT                   /note="SIT EcoNI"
FT   misc_binding    0..0
FT                   /note="SIT NruI"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli lacZ gene"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-HindIII, from pUC19"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT Eam1105I"
FT   misc_binding    0..0
FT                   /note="SIT Eco3I"
FT   misc_binding    0..0
FT                   /note="SIT ScaI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI lambda cos"
FT   misc_binding    0..0
FT                   /note="SIT EcoO109I"
FT   misc_binding    0..0
FT                   /note="SIT Psp5II"
FT   misc_binding    0..0
FT                   /note="SIT CvnI"
FT   CDS             complement(0..0)
FT                   /note="ANT strep gene"
FT   misc_binding    0..0
FT                   /note="SIT NspV"
FT   misc_binding    0..0
FT                   /note="SIT Eco72I"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT SstI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT TthI"
FT   misc_binding    0..0
FT                   /note="SIT NdeI"
FT   misc_binding    0..0
FT                   /note="SIT SapI"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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