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ID   PDF30      preliminary; circular DNA; SYN; 14000 BP.
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AC   ATCC37232;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Anacystis/E.coli plasmid vector pDF30 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pDF3 from A.nidulans; [pDF3 is similar to pUH24]
RC   pDF21, pDF30, pDF32 from pBR325 & pDF3
RA   Friedberg D., Seijffers J.;
RT   "A new hybrid plasmid capable of transforming Escherichia coli and
RT   Anacystis nidulans";
RL   Gene 22:267-275(1983).
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RN   [2]
RC   from Anacystis sp. 6311
RC   from O.limnetica
RA   Friedberg D., Seijffers J.;
RT   "Plasmids in two cyanobacteria strains";
RL   FEBS Lett. 107:165-168(1979).
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CC   Replicates, transforms, and confers ampicillin and chloramphenicol
CC   resistance in both Anacystis nidulans and E.coli.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pDF30)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli DR100)(Anacystis nidulans)(E.coli)(E.coli HB101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. A. nidulans 6311
FT                   -> pDF3 8000bp
FT                   1. pBR325 BamHI, tet gene
FT                   2. pDF3 BamHI, complete
FT                   -> pDF30"
FT   misc_binding    0..0
FT                   /note="SIT unique XhoI-SalI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//