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ID   PDG141     preliminary; circular DNA; SYN; 2500 BP.
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AC   ATCC39588;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pDG141 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pDG141 from pBR322 & trp promoter/operator
RA   ;
RT   ;
RL   Unpublished (1989).
RL   U.S. Patent No. 4,889,818 dated Dec. 26, 1989.
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RN   [2]
RC   pDG141 from pBR322 & trp promoter/operator
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,711,845 dated Dec. 12, 1987.
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RN   [3]
RC   pDG141 from pBR322 & trp promoter/operator
RA   ;
RT   ;
RL   Unpublished (1988).
RL   U.S. Patent No. 4,784,949 dated Nov. 15, 1988.
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CC   This is an expression vector with a tryptophan promoter-operator and
CC   ribosome binding site operably linked with an ATG start codon.[1]
CC   Restriction digests of the clone give the following sizes (kb):
CC   SacI--2.5; EcoRI--2.5. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pDG141)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322, ori/amp gene
FT                   2. E. coli, trp promoter/operator
FT                   -> pDG141 2500bp"
FT   misc_binding    0..0
FT                   /note="SIT unique SacI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli trp"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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