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ID   PDG1A      preliminary; circular DNA; SYN; 12200 BP.
AC   ATCC53005;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Aspergillus/E.coli cosmid vector pDG1 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pNE02 from pUC12 & Tn5
RC   pDG3 from pBB116 & pNE02
RC   pDG1 from pHC79 & pBB116, argB gene
RA   ;
RT   ;
RL   Unpublished (1989).
RL   U.S. Patent No. 4,855,249 dated Dec. 5, 1989.
RN   [2]
RC   pBB8 from pBR322 & pJDB219, yeast 2 micron/LEU2 gene
RC   pBB29 from pBR327 & pJDB219, yeast 2 micron/LEU2 gene
RC   pBB116 from pBB8 & A.nidulans argB gene
RC   pMS12 from pBB116
RA   Berse B., Dmochowska A., Skrzypek M., Weglenski P., Bates M.A.,
RA   Weiss R.L.;
RT   "Cloning and characterization of the ornithine
RT   carbamoyltransferase gene from Aspergillus nidulans";
RL   Gene 25:109-117(1983).
RN   [3]
RC   pJDB36 from yeast 2 micron A form & pMB9
RC   pJDB41 from yeast 2 micron B form & pMB9
RC   pJDB45 from yeast 2 micron B form & pMB9
RC   pJDB71 from yeast 2 micron A form & pMB9
RC   pJDB248 from yeast 2 micron A form & pMB9 & LEU2 gene
RC   pJDB219 from yeast 2 micron B form & pMB9 & LEU2 gene
RC   [pMB9-B1 from pMB9 & yeast 2 micron]
RA   Beggs J.D.;
RT   "Transformation of yeast by a replicating hybrid plasmid";
RL   Nature 275:104-109(1978).
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI/HindIII--9.4,1.5,1.3; EcoRI/BamHI--6.3, 5.9; PstI--10.2, 2.0.
CC   (ATCC staff)
CC   The argB BamHI/EcoRI fragment contains the following sites (separated
CC   by approximate kb): BamHI - 0.8 - SalI - 0.4 - HindIII - 1.4 - SalI
CC   - 0.1 - HindIII - 0.7 - SalI - 0.1 - HindIII - 0.7 - SalI - 0.7 -
CC   SalI - 0.5 - EcoRI. [2]
CC   This was constructed by inserting the 5.0 kb EcoRI/HindIII fragment
CC   from pBB116 containing argB into pHC79 cut with EcoRI + HindIII. [1]
CC   This is an integrative, positive selection vector conferring arginine
CC   prototrophy on auxotrophic host such as Aspergillus niger 350(-)52
CC   (ATCC 20739) (argB-). [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pDG1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   HO (E.coli HB101)(Aspergillus niger)(E.coli)
CC   HO (Aspergillus niger 350-52 [ATCC 20739])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pMB8
FT                   -> pMB9 5300bp
FT                   1. pMB9 EcoRI, 5' to tet gene
FT                   2. yeast 2 micron B form EcoRI-EcoRI 2100bp, ori
FT                   -> pJBD41 [tet gene]
FT                   1. pJBD41 PstI, amp gene
FT                   dAT tailing
FT                   2. yeast 1200bp, LEU2 gene
FT                   sheared
FT                   dAT tailing
FT                   -> pJDB219
FT                   1. pBR322 EcoRI
FT                   2. pJDB219 EcoRI-EcoRI, yeast 2 micron/LEU2 gene
FT                   -> pBB8 11400bp
FT                   1. pBB8 BamHI
FT                   2. A.nidulans BamHI-BamHI 6200bp, argB gene
FT                   -> pBB116
FT                   1. pHC79
FT                   2. pBB116 SalI-SalI, argB gene
FT                   -> pDG1"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="GEN Aspergillus ornithine carbamoyltransferase
FT                   gene (argB)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;