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ID   PDG3       preliminary; circular DNA; SYN; 7800 BP.
AC   ATCC53006;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   Aspergillus/E.coli plasmid vector pDG3 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pNE02 from pUC12 & Tn5
RC   pDG3 from pBB116 & pNE02
RC   pDG1 from pHC79 & pBB116, argB gene
RA   ;
RT   ;
RL   Unpublished (1989).
RL   U.S. Patent No. 4,855,249 dated Dec. 5, 1989.
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII/SalI--3.1, 1.65, 1.3, 0.75, 0.5 + smaller; XbaI--4.4, 3.4;
CC   EcoRI--7.8; SmaI--4.3, 3.5; SphI--7.8. (ATCC staff)
CC   This was constructed by inserting a HindIII/SalI fragment of Tn5 into
CC   pUC12 cut with HindIII/SalI (pNE02) and then inserting an XbaI
CC   fragment from pBB116 into the XbaI site of pNE02. [1]
CC   This is an integrative, selectable vector conferring arginine
CC   prototrophy on arginine autotrophic hosts such as Aspergillus niger
CC   350(-)52, ATCC 20739 (argB-). It also confers kanamycin resistance.[1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pDG3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli JM83)(Aspergillus niger)(E.coli)
CC   HO (Aspergillus niger 350-52 [ATCC 20739])
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC12 remove HindIII-SalI, MCS
FT                   2. Tn5 HindIII-SalI
FT                   -> pNE02
FT                   1. pBB116 XbaI-XbaI
FT                   2. pNE02 XbaI
FT                   -> pDG3"
FT   misc_binding    0..0
FT                   /note="MCS BamHI-SmaI-SstI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-SmaI-SphI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli neomycin phosphotransferase gene
FT                   (NPT); neomycin resistance gene (neo)"
FT   CDS             0..0
FT                   /note="GEN Aspergillus ornithine carbamoyltransferase
FT                   gene (argB)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;