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ID   PDGR2      preliminary; circular DNA; SYN; 10400 BP.
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AC   ATCC53100;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Vertebrate/E.coli plasmid vector pDGR-2 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pDGR-2 from pBR322 & SV40
RA   ;
RT   ;
RL   Unpublished (1989).
RL   U.S. Patent No. 4,868,112, 1989.
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CC   This vector replicates in COS-1 African green monkey kidney cells as
CC   well as in E.coli. (personal communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--5.3,3.2,1.9; BamHI--3.5, 2.4 doublet, 1.2, 0.8;
CC   HindIII--7.0, 1.4, 0.7, 0.6. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pDGR-2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)(monkey COS-1 cell line)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. SV40, ori
FT                   -> pDGR-2 10400bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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