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ID   PDGUSBIN19 preliminary; circular DNA; SYN; 5347 BP.
XX
AC   U12638;
XX
DT   25-AUG-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli vector pdeltagusBin19 - incomplete, T-DNA region.
XX
KW   cloning vector; pdeltagusBin19; T-DNA; neomycin phosphotransferase;
KW   promoter-trapping; beta-glucuronidase.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-5347
RC   pdeltagusBin19 from pBIN19
RA   Wei W., McArdle H., Lindsey K.;
RT   "T-DNA sequence of the promoter-trapping vector pdeltagusBin19";
RL   Unpublished (1994).
XX
RN   [2]
RC   pdeltagusBin19 from pBIN19
RA   Topping J.F., Wei W., Lindsey K.;
RT   "Functional tagging of regulatory elements in the plant genome";
RL   Development 112:1009-1019(1991).
XX
RN   [3]
RP   1-5347
RC   pdeltagusBin19
RA   Wei W.;
RT   ;
RL   Submitted (22-JUL-1994) by:
RL   Wei W., Botany, University of Leicester, University Road,
RL   Leicester, LE1 7RH, UK.
XX
CC   NCBI gi: 529325
CC   NM (pdeltagusBin19)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli, beta-glucuronidase gene
FT                   -> pRAJ260
FT                   1. pBIN19 remove 8200bp, 3600bp
FT                   2. pRAJ260 1800bp, E. coli beta-glucuronidase gene
FT                   -> pdeltagusBin19 5347bp"
FT   repeat_unit     1..25
FT                   /note="the left border repeat"
FT   misc_feature    1..78
FT                   /note="the left border sequence of pTiT37"
FT   misc_feature    1..664
FT                   /note="pBin19 sequence"
FT   misc_feature    79..508
FT                   /note="M13mp19 intergenic region"
FT   misc_feature    509..664
FT                   /note="M13mp19 sequence"
FT   CDS             684..2492
FT                   /note="GEN E. coli beta-glucuronidase gene,
FT                   from pRAJ260 [3]"
FT   3'UTR           2563..2815
FT                   /note="nopaline synthase gene 3'UTR"
FT   misc_feature    2846..5347
FT                   /note="ANT E. coli neomycin phosphotransferse gene
FT                   (NPT), driven by nos promoter"
FT   CDS             complement(4168..4962)
FT                   /note="ANT E. coli neomycin phosphotransferse gene
FT                   (NPT), neomycin resistance gene (neo)"
FT   repeat_unit     5323..5347
FT                   /note="the right border repeat"
XX
SQ   Sequence 5347 BP; 1371 A; 1345 C; 1419 G; 1212 T; 0 other;
     tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
     gacgttttta atgtactggg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     gcaggcgaaa atcctgtttg atggtggttc cgaaatcggc aaaatccctt ataaatcaaa
     agaatagccc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     ggaagggaag aaagcgaaag gagcgggcgc cattcaggct gcgcaactgt tgggaagggc
     gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
     gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
     aattcccggg tggtcagtcc cttatgttac gtcctgtaga aaccccaacc cgtgaaatca
     aaaaactcga cggcctgtgg gcattcagtc tggatcgcga aaactgtgga attgatcagc
     gttggtggga aagcgcgtta caagaaagcc gggcaattgc tgtgccaggc agttttaacg
     atcagttcgc cgatgcagat attcgtaatt atgcgggcaa cgtctggtat cagcgcgaag
     tctttatacc gaaaggttgg gcaggccagc gtatcgtgct gcgtttcgat gcggtcactc
     attacggcaa agtgtgggtc aataatcagg aagtgatgga gcatcagggc ggctatacgc
     catttgaagc cgatgtcacg ccgtatgtta ttgccgggaa aagtgtacgt atcaccgttt
     gtgtgaacaa cgaactgaac tggcagacta tcccgccggg aatggtgatt accgacgaaa
     acggcaagaa aaagcagtct tacttccatg atttctttaa ctatgccgga atccatcgca
     gcgtaatgct ctacaccacg ccgaacacct gggtggacga tatcaccgtg gtgacgcatg
     tcgcgcaaga ctgtaaccac gcgtctgttg actggcaggt ggtggccaat ggtgatgtca
     gcgttgaact gcgtgatgcg gatcaacagg tggttgcaac tggacaaggc actagcggga
     ctttgcaagt ggtgaatccg cacctctggc aaccgggtga aggttatctc tatgaactgt
     gcgtcacagc caaaagccag acagagtgtg atatctaccc gcttcgcgtc ggcatccggt
     cagtggcagt gaagggccaa cagttcctga ttaaccacaa accgttctac tttactggct
     ttggtcgtca tgaagatgcg gacttacgtg gcaaaggatt cgataacgtg ctgatggtgc
     acgaccacgc attaatggac tggattgggg ccaactccta ccgtacctcg cattaccctt
     acgctgaaga gatgctcgac tgggcagatg aacatggcat cgtggtgatt gatgaaactg
     ctgctgtcgg ctttaacctc tctttaggca ttggtttcga agcgggcaac aagccgaaag
     aactgtacag cgaagaggca gtcaacgggg aaactcagca agcgcactta caggcgatta
     aagagctgat agcgcgtgac aaaaaccacc caagcgtggt gatgtggagt attgccaacg
     aaccggatac ccgtccgcaa gtgcacggga atatttcgcc actggcggaa gcaacgcgta
     aactcgaccc gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg
     ataccatcag cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc
     aaagcggcga tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg
     agaaactgca tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc
     actcaatgta caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc
     accgcgtctt tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt
     ttgcgacctc gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg
     accgcaaacc gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg
     gtgaaaaacc gcagcaggga ggcaaacaat gaatcaacaa ctctcctggc gcaccatcgt
     cggctacagc ctcgggaatt gctaccgagc tcgaatttcc ccgatcgttc aaacatttgg
     caataaagtt tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt
     ctgttgaatt acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga
     tgggttttta tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata
     tagcgcgcaa actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa
     ttggggatcc gtcgacctgc agccaagctt ggcgtaatca tggtcatagc tgtttcctgt
     gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     aggcggtttg cgtattgggc caaagacaaa agggcgacat tcaaccgatt gagggaggga
     aggtaaatat tgacggaaat tattcattaa aggtgaatta tcaccgtcac cgacttgagc
     catttgggaa ttagagccag caaaatcacc agtagcacca ttaccattag caaggccgga
     aacgtcacca atgaaaccat cgatagcagc accgtaatca gtagcgacag aatcaagttt
     gcctttagcg tcagactgta gcgcgttttc atcggcattt tcggtcatag cccccttatt
     agcgtttgcc atcttttcat aatcaaaatc accggaacca gagccaccac cggaaccgcc
     tccctcagag ccgccaccct cagaaccgcc accctcagag ccaccaccct cagagccgcc
     accagaacca ccaccagagc cgccgccagc attgacagga ggccccgatc tagtaacata
     gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt ttctatcgcg
     tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa taacgtcatg
     cattacatgt taattattac atgcttaacg taattcaaca gaaattatat gataatcatc
     gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt tgaacgatcg
     gggatcatcc gggtctgtgg cgggaactcc acgaaaatat ccgaacgcag caagatatcg
     cggtgcatct cggtcttgcc tgggcagtcg ccgccgacgc cgttgatgtg gacgccgggc
     ccgatcatat tgtcgctcag gatcgtggcg ttgtgctcgt cggccgttgc tgtcgtaatg
     atatcggcac cttcgaccgc ctgttccgca gagatccctg ggcgaagaac tccagcatga
     gatccccgcg ctggaggatc atccagccgg cgtcccggaa aacgattccg aagcccaacc
     tttcatagaa ggcggcggtg gaatcgaaat ctcgtgatgg caggttgggc gtcgcttggt
     cggtcatttc gaaccccaga gtcccgctca gaagaactcg tcaagaaggc gatagaaggc
     gatgcgctgc gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc
     gccgccaagc tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc
     cacacccagc cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt
     cggcaagcag gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt
     gagcctggcg aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg
     atcgacaaga ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg
     gtcgaatggg caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat
     ggatactttc tcggcaggag caaggtgaga tgacaggaga tcctgccccg gcacttcgcc
     caatagcagc cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac
     gcccgtcgtg gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc
     ggacaggtcg gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc
     ggcatcagag cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca
     agcggccgga gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atccagatcc
     ggtgcagatt atttggattg agagtgaata tgagactcta attggatacc gaggggaatt
     tatggaacgt cagtggagca tttttgacaa gaaatatttg ctagctgata gtgaccttag
     gcgacttttg aacgcgcaat aatggtttct gacgtatgtg cttagctcat taaactccag
     aaacccgcgg ctgagtggct ccttcaatcg ttgcggttct gtcagttcca aacgtaaaac
     ggcttgtccc gcgtcatcgg cgggggtcat aacgtgactc ccttaattct ccgctcatga
     tcagattgtc gtttcccgcc ttcagtttaa actatcagtg tttgacagga tatattggcg
     ggtaaac
//