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ID   PDK1       preliminary; circular DNA; SYN; 12500 BP.
XX
AC   ATCC37519;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
XX
DE   Pseudomonas plasmid vector pDK1 - incomplete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pDK1 from P.putida HS1 TOL plasmids
RC   pDKC1, pDKM1, pDKT1 from pDK1
RA   Kunz D.A., Chapman P.J.;
RT   "Isolation and characterization of spontaneously occurring TOL
RT   plasmid mutants of Pseudomonas putida HS1";
RL   J. Bacteriol. 146:952-964(1981).
XX
CC   Plasmid confers ability to degrade toluene, m- and p-xylene,
CC   1,2,4-trimethyl benzene (pseudocumene), and 3-ethyltoluene.
CC   The phenotype is Ben+ Mtol+ Ptol+ Tln+ Mxy+ Pxy+. The genotype is
CC   w+/TOL(pDK1).
CC   Medium is 1065 LB medium.
CC   NM (pDK1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Pseudomonas putida HS1 [PpC1])(Pseudomonas)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. P.putida HS1 TOL plasmids, tol
FT                   -> pDK1"
FT   rep_origin      0..0
FT                   /note="ORI Pseudomonas pDK1"
XX
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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