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ID   PDK101     preliminary; circular DNA; SYN; 3027 BP.
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AC   ATCC77406;
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DT   03-FEB-1994 (Rel. 8, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pDK101 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   from pGEM-5Zf+ & oligo
RA   Kovalic D., Kwak J.H., Weisblum B.;
RT   "General method for direct cloning of DNA fragments generated by
RT   the polymerase chain reaction";
RL   Nucleic Acids Res. 19:4560-4560(1991).
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CC   Deposited by: Bernard Weisblum
CC   Restriction digests of the clone give the following sizes (kb):
CC   BglI--1.7, 1.3; EcoRV--3.0; PstI--3.0. (ATCC staff)
CC   Vector useful for cloning-double stranded amplification products.
CC   Permits synthesis of ssDNA and contains promoters for in vitro RNA
CC   synthesis. [1]
CC   The plasmid contains the following restriction sites (bp from nt 1):
CC   BglI--1533, 2854; EcoRV--73; NcoI--37, 61; NdeI--105; NotI--85;
CC   PstI--93; PvuI--1786, 2885; PvuII--348, 2915; SacI--114; SalI--99;
CC   XcmI--44, 59; XmnI--2014. [1]
CC   The order of the major features in this plasmid is:
CC   T7/ApaI/MCS/NsiI/lacZ'SP6 - pMB1 ori - pMB1 - ampR - f1 ori. (personal
CC   communication)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pDK101)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli)(E.coli JM109)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-5Zf+ 3003bp
FT                   -> pDK101 3027bp"
FT   misc_binding    0..0
FT                   /note="MCS ApaI-AatII-SphI-XcmI-BalI-XcmI-SacII-
FT                   EcoRV-SpeI-NotI-PstI-AccI-HincII-SalI-NdeI-
FT                   SacI-BstXI-MluI-NsiI"
FT   misc_binding    0..0
FT                   /note="SIT unique XcmI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   promoter        0..0
FT                   /note="PRO E. coli lac"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   promoter        0..0
FT                   /note="PRO bacteriophage SP6"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ');
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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