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ID   PDM2       preliminary; circular DNA; SYN; 9900 BP.
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AC   ATCC37136;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
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DE   Haemophilus/E.coli plasmid vector pDM2 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pDM2 from RSF0885 & p2265
RA   McCarthy D., Clayton N.L., Setlow J.K.;
RT   "A plasmid cloning vehicle for Haemophilus influenzae and
RT   Escherichia coli";
RL   J. Bacteriol. 151:1605-1607(1982).
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RN   [2]
RC   [p(amp90383) from H.influenzae Rd]
RC   p1002 from p(amp90383) & H.influenzae Rd, amp gene/tet gene
RC   p2193 from p(amp90383) & H.influenzae Rd, tet gene/cat gene
RC   p2265 from p(amp90383) & H.influenzae Rd, tet gene/cat gene
RC   p2365 from p(amp90383) & H.influenzae Rd, tet gene/cat gene
RC   p2316 from p(amp90383) & H.influenzae Rd, tet gene/cat gene
RA   Albritton W.L., Bendler J.W., Setlow J.K.;
RT   "Plasmid transformation in Haemophilus influenzae";
RL   J. Bacteriol. 145:1099-1101(1981).
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RN   [3]
RC   pUB701 from Haemophilus influenzae type b
RC   RSF007 from Haemophilus influenzae type b
RA   Elwell L.P., Saunders J.R., Richmond M.H., Falkow S.;
RT   "Relationships among some R plasmids found in Haemophilus
RT   influenzae";
RL   J. Bacteriol. 131:356-362(1977).
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RN   [4]
RC   pJB1 from Haemophilus influenzae
RA   Brunton J.L., Maclean I.W., Albritton W.L.;
RT   "Plasmid-mediated ampicillin resistance in Haemophilus ducreyi";
RL   Antimicrob. Agents Chemother. 15:294-299(1979).
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CC   Incubate with 3-8% carbon dioxide.  This strain takes several days to
CC   grow up.
CC   A vector for cloning in Haemophilus influenza. (ATCC staff)
CC   Medium is 814 GC medium.
CC   NM (pDM2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Haemophilus influenzae rec-1)(Haemophilus influenzae)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli
FT                   -> RSF0885 5700bp
FT                   1. H.influenzae Rd
FT                   -> p(amp90383)
FT                   1. p(amp90383)
FT                   2. H.influenzae Rd, tet gene/cat gene
FT                   -> p2265 34000bp
FT                   1. RSF0885 PvuII
FT                   2. p2265 PvuII
FT                   -> pDM2 9800bp"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI-BstEII-XbaI-PstI-PvuI-BglI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pRSF0885"
FT   rep_origin      0..0
FT                   /note="ORI Haemophilus p2265"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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