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ID   PDS20TRP   preliminary; circular DNA; SYN; 5180 BP.
AC   M34334;
DT   15-SEP-1990 (Rel. 5, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pDS20TRP - incomplete, tryptophan promoter.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-74
RC   pDS20 from pKG1800
RC   pDS20TRP from pDS20
RC   pXL534 from pXL276
RC   pXL670 from pXL534
RC   pXL879 from pXL534 & pKRS101
RC   pXL712 from pDR720 & pXL534
RC   pXL717 from pRPG9 & pXL534
RC   pXL730 from pRPG9 & pXL712
RC   pXL1077 from pRPG9 & pXL879
RC   pXL1741 from pXL305
RC   pXL1743 from pXL534
RC   pXL1745 from pXL717
RC   pXL305 from pDS20 & pXL276
RC   pXL814, pXL892 from pXL879 & pXL305
RC   pXL1312, pXL1313 from pDR720 & pXL305
RA   Latta M., Philit M., Maury I., Soubrier F., Denefle P., Mayaux J.F.;
RT   "Tryptophan promoter derivatives on multicopy plasmids: a
RT   comparative analysis of expression potentials in Escherichia coli";
RL   DNA Cell Biol. 9:129-137(1990).
CC   pDS20 has 5180 bp.
CC   NCBI gi: 208327
CC   NM (pDS20TRP)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA (pDS20)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pKG1800 4900bp
FT                   -> pDS20 5100bp
FT                   1. pDS20 remove EcoRI-SalI 5100bp
FT                   2. trp promoter EcoRI-SalI 78bp, -77 to +1
FT                   synthetic 95bp
FT                   -> pDS20TRP 5180bp"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   promoter        0..0
FT                   /note="PRO gal"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   misc_binding    0..0
FT                   /note="SIT SmaI"
FT   CDS             0..0
FT                   /note="GEN galK gene"
FT   misc_binding    0..0
FT                   /note="SIT HpaI"
FT   misc_binding    0..0
FT                   /note="SIT HpaI"
FT   misc_binding    0..0
FT                   /note="SIT BamHI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (amp/apr)"
SQ   Sequence 74 BP; 18 A; 18 C; 15 G; 23 T; 0 other;
     ctcaaggcgc actcccgttc tggataatgt tttttgcgcc gacatcataa cggttctggc
     aaatattctg aaat