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ID   PDS5       preliminary; circular DNA; SYN; 105 BP.
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AC   IG5124;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pDS5 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pDS5 from pUC8 & T5
RC   pDS6 from pDS5 & lambda, terminator t0
RC   pDS7 or pDS5/2 from pDS5 & DHFR gene
RC   pDS8 from pDS5 & DHFR gene & lambda, terminator t0
RC   pDS9 from pDS5 & DHFR gene & RBS
RC   pDS10 or pDS5/3 from pDS5 & DHFR gene & lambda, terminator t0/RBS
RC   pDS20 from pDS5 & lacI gene & lambda, terminator t0
RC   pDS21 from pDS5 & DHFR gene & RBS & lacI gene
RC   pDS30 from pDS5 & phage f1 ori
RC   pDS31 from pDS5 & phage f1 ori & lambda, terminator t0
RC   pDS32 from pDS5 & lacI gene & phage f1 ori & lambda, terminator t0
RC   pUHE22 from pUC8 & T5
RC   pUHE24 from pUC8 & T5
RC   pUHE25 from pUC8 & T5
RA   Bujard H., Gentz R., Lanzer M., Stueber D., Muller H.M., Ibrahimi I.,
RA   Hauptle M.T., Dobberstein B.;
RT   "A T5 promoter-based transcription-translation system for the
RT   analysis of proteins in vitro and in vivo";
RL   Meth. Enzymol. 155:416-433(1987).
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RN   [2]
RC   [pN25x/o from E.coli phage T5 pN25 promoter & E. coli lac operator]
RC   pDS5, pDS6 from pDS1 & pUC8 & rrnB terminator t1 & pN25x/o
RC   pDS5/1 from pDS5
RC   pDS5/2 from pDS5 & DHFR gene
RC   pDS5/3 from pDS5/2 & RBS
RC   pDS5/4 from pDS5/1 & chicken lysozyme cDNA
RA   Stueber D., Ibrahimi I., Cutler D., Dobberstein B., Bujard H.;
RT   "A novel in vitro transcription-translation system: accurate and
RT   efficient synthesis of single proteins from cloned DNA sequences";
RL   EMBO J. 3:3143-3148(1984).
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RN   [3]
RC   pDS1 from pBR322 & DHFR gene & cat gene
RC   pDS2 from pBR322 & DHFR gene & cat gene
RA   Stueber D., Bujard H.;
RT   "Transcription from efficient promoters can interfere with plasmid
RT   replication and diminish expression of plasmid specified genes";
RL   EMBO J. 1:1399-1404(1982).
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CC   MCS. promoter. not original sequence.
CC   NM (pDS5)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC8)(pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E.coli phage T5 pN25 promoter
FT                   2. E. coli lac operator
FT                   -> pN25x/o
FT                   1. pBR322 PvuII-EcoRI 2293bp 2067..4360, ori/amp gene
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   3. DHFR gene
FT                   -> pDS1
FT                   1. pDS1
FT                   2. pN25x/o
FT                   -> plasmid
FT                   1. plasmid
FT                   2. pUC8 EcoRI-HindIII 30bp 231..261
FT                   -> plasmid2
FT                   1. plasmid2
FT                   2. E. coli rrnB terminator t1
FT                   -> pDS5"
FT   misc_binding    0..0
FT                   /note="SIT XhoI"
FT   promoter        0..0
FT                   /note="PRO E.coli phage T5 pN25 promoter"
FT   promoter        0..0
FT                   /note="PRO E. coli lac operator"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-HindIII, from pUC8"
FT   CDS             0..0
FT                   /note="ANT E. coli cat gene"
FT   terminator      0..0
FT                   /note="TER E. coli rrnB T1"
FT   misc_binding    0..0
FT                   /note="SIT XbaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   misc_binding    0..0
FT                   /note="SIT AatII"
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SQ   Sequence 105 BP; 32 A; 18 C; 21 G; 34 T; 0 other;
     aaaaatttat ttgctttcag gaaaattttt ctgtataata gattcaaatt gtgagcggat
     aacaatttga attcccgggg atccgtcgac ctgcagccaa gcttg
//