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ID   PDSP11     preliminary; circular DNA; SYN; 6993 BP.
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AC   M12159;
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DT   18-NOV-1986 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pDSP1 seg 1 - incomplete, segment 1, galK gene.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   382-409
RC   pSVKgpt from pSVK
RC   pSVKgpt* from pSVKgpt
RC   pSVK3gpt from pSVKgpt & pYSK9-12 & linker
RC   pMLRg*s from pSVK & pSVKgpt*
RC   pKSVgpt*N- from pMLRg*s
RC   pDSP1 from pKSVgpt*N- & pSVK3gpt
RA   Pfarr D.S., Sathe G., Reff M.E.;
RT   "Laboratory methods: A highly modular cloning vector for the
RT   analysis of eukaryotic genes and gene regulatory elements";
RL   DNA 4:461-467(1985).
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RN   [2]
RP   1-2553
RC   pDSP1
RA   Reff M.E.;
RT   ;
RL   Submitted (29-MAY-1986) to GenBank on computer-readable media by:
RL   Reff M.E., Smith Kline and French Labs, Swedeland PA 19479.
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RN   [3]
RC   pMLRg*s from pSVK & pSVKgpt*
RA   Pfarr D.S., Rieser L.A., Woychik R.P., Rottman F.M., Rosenberg M.,
RA   Reff M.E.;
RT   "Differential effects of polyadenylation regions on gene
RT   expression in mammalian cells";
RL   DNA 5:115-122(1986).
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RN   [4]
RC   pSVKgpt from pSVK
RA   Johansen H., Reff M., Schumperli D., Rosenberg M.;
RT   "Studying eukaryotic transcription and translation by gene fusion";
RL   Alfred Benzon Symposium 19:413-429(1984).
RL   Clark B.F.C., Petersen H., eds., Munksgard, Copenhagen.
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RN   [5]
RC   pSVK100 from pDSdeltaK & pSV2-gpt
RC   pSVK200 from pDSdeltaK & pSV2-gpt
RC   pSVK300 from pDSdeltaK & pSV2-gpt
RC   pSVK400 from pDSdeltaK & pSV2-gpt
RC   pSVK500 from pDSdeltaK & pSV2-gpt
RC   pSVK150, pSVK160 from pSVK100 & linker
RC   pSVK250, pSVK260 from pSVK200 & linker
RC   pSVK350, pSVK360 from pSVK300 & linker
RC   pSVK151, pSVK152 from pSVK150
RA   Johansen H., Schumperli D., Rosenberg M.;
RT   "Affecting gene expression by altering the length and sequence
RT   of the 5' leader";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:7698-7702(1984).
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RN   [6]
RC   pYSK9-12 from yeast galK gene
RA   Clark B.F.C.;
RT   ;
RL   Unpublished (1985).
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CC   Vector pDSP1 contains two independent mammalian transcription
CC   cassettes containing SV40 early gene regulatory elements
CC   controlling either the E. coli galK or xgprt genes.
CC   The regulatory elements of the galK cassette are
CC   bounded by multiple restriction sites allowing for easy removal and
CC   replacement of the regulatory sequences or the galK gene itself.
CC   NCBI gi: 208227
CC   NM (pDSP1 seg 1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (promoter analysis)(expression eukaryote)
CC   SE (color red/white)
CC   PA (pSVK3gpt from pSVKgpt)(pKSVgpt*N from pMLRg*s)
CC   BR (pDSP1 seg 2)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
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FT   misc_feature    0..0
FT                   /note="1. yeast galK gene
FT                   -> pYSK9-12
FT                   1. E. coli galK gene
FT                   2. E. coli gpt gene
FT                   -> pSVK
FT                   1. pSVK
FT                   2. xgprt gene BamHI-BamHI
FT                   -> pSVKgpt
FT                   1. pSVKgpt remove HindIII-BglII 116bp, xgprt 5' leader
FT                   -> pSVKgpt*
FT                   1. pSVKgpt remove HindIII-MluI, SV40 and galK gene
FT                   2. linker HindIII-blunt end 6bp aagctt
FT                   3. pYSK9-12 EcoRI-MluI, galK gene
FT                   mung bean nuclease:
FT                   -> pSVK3gpt
FT                   1. pSVK NdeI, in pBR322 2299
FT                   fill in
FT                   2. pSVKgpt* BamHI-BamHI, xgprt gene
FT                   fill in:fill in
FT                   -> plasmid
FT                   1. plasmid remove HpaI-HpaI, SV40 t splice
FT                   HindIII linker 6bp aagctt
FT                   -> plasmid2
FT                   1. plasmid2 PvuII, SV40 early promoter
FT                   2. M13 34bp, MCS
FT                   -> pMLRg*s
FT                   1. pMLRg*s HindIII, SV40 polyA
FT                   fill in
FT                   MCS linker 34bp, M13 MCS
FT                   BamHI, xgprt gene
FT                   fill in
FT                   MCS linker 34bp, M13 MCS
FT                   -> plasmid3
FT                   1. plasmid3 NcoI, SV40 early promoter
FT                   fill in
FT                   NcoI, SV40 early promoter
FT                   fill in
FT                   -> pKSVgpt*N
FT                   1. pSVK3gpt SfiI-MluI
FT                   2. pKSVgpt*N remove small SfiI-MluI
FT                   -> pDSP1 6993bp"
FT   misc_recomb     <1..>1
FT                   /note="pBR322 DNA end/M13 DNA start"
FT   misc_recomb     38..39
FT                   /note="M13 DNA end/SV40 early promoter start"
FT   promoter        39..384
FT                   /note="PRO SV40 early genes"
FT   misc_recomb     384..385
FT                   /note="SV40 early promoter DNA end/synthetic linker
FT                   start"
FT   CDS             395..1543
FT                   /note="GEN E. coli galactokinase gene (galK);
FT                   AA 5 is at 407"
FT   misc_recomb     406..407
FT                   /note="synthetic linker end/E. coli galK cds start
FT                   (AA 5)"
FT   misc_recomb     1589..1590
FT                   /note="E. coli galK DNA end/synthetic linker start"
FT   misc_binding    1590..1641
FT                   /note="MCS"
FT   misc_recomb     1641..1642
FT                   /note="synthetic linker end/SV40 DNA sart"
FT   misc_recomb     1776..1777
FT                   /note="SV40 DNA end/synthetic linker start"
FT   misc_binding    1777..1800
FT                   /note="MCS"
FT   misc_recomb     1800..1801
FT                   /note="synthetic linker end/SV40 late region DNA
FT                   start"
FT   CDS             395..1543
FT                   /note="GEN E. coli galactokinase gene (galK);
FT                   NCBI gi: 208230"
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SQ   Sequence 2553 BP; 653 A; 666 C; 601 G; 633 T; 0 other;
     agcttggctg caggtcgacg gatcgatccc cgggaattct gtggaatgtg tgtcagttag
     ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     ctccgcccag ttccgcccat tctccgcccc atgcatggct gactaatttt ttttatttat
     gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     ggaggcctag gcttttgcaa aaagcttggc caccatggct ctccaagaaa aaacacaatc
     tctgtttgcc aacgcatttg gctaccctgc cactcacacc attcaggcgc ctggccgcgt
     gaatttgatt ggtgaacaca ccgactacaa cgacggtttc gttctgccct gcgcgattga
     ttatcaaacc gtgatcagtt gtgcaccacg cgatgaccgt aaagttcgcg tgatggcagc
     cgattatgaa aatcagctcg acgagttttc cctcgatgcg cccattgtcg cacatgaaaa
     ctatcaatgg gctaactacg ttcgtggcgt ggtgaaacat ctgcaactgc gtaacaacag
     cttcggcggc gtggacatgg tgatcagcgg caatgtgccg cagggtgccg ggttaagttc
     ttccgcttca ctggaagtcg cggtcggaac cgtattgcag cagctttatc atctgccgct
     ggacggcgca caaatcgcgc ttaacggtca ggaagcagaa aaccagtttg taggctgtaa
     ctgcgggatc atggatcagc taatttccgc gctcggcaag aaagatcatg ccttgctgat
     cgattgccgc tcactgggga ccaaagcagt ttccatgccc aaaggtgtgg ctgtcgtcat
     catcaacagt aacttcaaac gtaccctggt tggcagcgaa tacaacaccc gtcgtgaaca
     gtgcgaaacc ggtgcgcgtt tcttccagca gccagccctg cgtgatgtca ccattgaaga
     gttcaacgct gttgcgcatg aactggaccc gatcgtggca aaacgcgtgc gtcatatact
     gactgaaaac gcccgcaccg ttgaagctgc cagcgcgctg gagcaaggcg acctgaaacg
     tatgggcgag ttgatggcgg agtctcatgc ctctatgcgc gatgatttcg aaatcaccgt
     gccgcaaatt gacactctgg tagaaatcgt caaagctgtg attggcgaca aaggtggcgt
     acgcatgacc ggcggcggat ttggcggctg tatcgtcgcg ctgatcccgg aagagctggt
     gcctgccgta cagcaagctg tcgctgaaca atatgaagca aaaacaggta ttaaagagac
     tttttacgtt tgtaaaccat cacaaggagc aggacagtgc tgaacgaaac tcccgcactg
     gcacccgatg gtcagccgta ccgactgttc aagctagaat tctctagagc tcgctgatca
     gcctcgaggc cagctagctt gaacttgttt attgcagctt ataatggtta caaataaagc
     aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     tccaaactca tcaatgtatc ttatcatgtc tggatccctc gaggggggat cccgtcgacc
     gatccccagg aagctcctct gtgtcctcat aaaccctaac ctcctctact tgagaggaca
     ttccaatcat aggctgccca tccaccctct gtgtcctcct gttaattagg tcacttaaca
     aaaaggaaat tgggtagggg tttttcacag accgctttct aagggtaatt ttaaaatatc
     tgggaagtcc cttccactgc tgtgttccag aagtgttggt aaacagccca caaatgtcaa
     cagcagaaac atacaagctg tcagctttgc acaagggccc aacaccctgc tcatcaagaa
     gcactgtggt tgctgtgtta gtaatgtgca aaacaggagg cacattttcc ccacctgtgt
     aggttccaaa atatctagtg ttttcatttt tacttggatc aggaacccag cactccactg
     gataagcatt atccttatcc aaaacagcct tgtggtcagt gttcatctgc tgactgtcaa
     ctgtagcatt ttttggggtt acagtttgag caggatattt ggtcctgtag tttgctaaca
     caccctgcag ctccaaaggt tccccaccaa cagcaaaaaa atgaaaattt gacccttgaa
     tgggttttcc agcaccattt tcatgagttt tttgtgtccc tgaatgcaag tttaacatag
     cagttacccc aataacctca gttttaacag taacagcttc ccacatcaaa atatttccac
     aggttaagtc ctcatttaaa ttaggcaaag gaa
//