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ID PDSP11 preliminary; circular DNA; SYN; 6993 BP.
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AC M12159;
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DT 18-NOV-1986 (Rel. 1, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli plasmid vector pDSP1 seg 1 - incomplete, segment 1, galK gene.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 382-409
RC pSVKgpt from pSVK
RC pSVKgpt* from pSVKgpt
RC pSVK3gpt from pSVKgpt & pYSK9-12 & linker
RC pMLRg*s from pSVK & pSVKgpt*
RC pKSVgpt*N- from pMLRg*s
RC pDSP1 from pKSVgpt*N- & pSVK3gpt
RA Pfarr D.S., Sathe G., Reff M.E.;
RT "Laboratory methods: A highly modular cloning vector for the
RT analysis of eukaryotic genes and gene regulatory elements";
RL DNA 4:461-467(1985).
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RN [2]
RP 1-2553
RC pDSP1
RA Reff M.E.;
RT ;
RL Submitted (29-MAY-1986) to GenBank on computer-readable media by:
RL Reff M.E., Smith Kline and French Labs, Swedeland PA 19479.
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RN [3]
RC pMLRg*s from pSVK & pSVKgpt*
RA Pfarr D.S., Rieser L.A., Woychik R.P., Rottman F.M., Rosenberg M.,
RA Reff M.E.;
RT "Differential effects of polyadenylation regions on gene
RT expression in mammalian cells";
RL DNA 5:115-122(1986).
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RN [4]
RC pSVKgpt from pSVK
RA Johansen H., Reff M., Schumperli D., Rosenberg M.;
RT "Studying eukaryotic transcription and translation by gene fusion";
RL Alfred Benzon Symposium 19:413-429(1984).
RL Clark B.F.C., Petersen H., eds., Munksgard, Copenhagen.
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RN [5]
RC pSVK100 from pDSdeltaK & pSV2-gpt
RC pSVK200 from pDSdeltaK & pSV2-gpt
RC pSVK300 from pDSdeltaK & pSV2-gpt
RC pSVK400 from pDSdeltaK & pSV2-gpt
RC pSVK500 from pDSdeltaK & pSV2-gpt
RC pSVK150, pSVK160 from pSVK100 & linker
RC pSVK250, pSVK260 from pSVK200 & linker
RC pSVK350, pSVK360 from pSVK300 & linker
RC pSVK151, pSVK152 from pSVK150
RA Johansen H., Schumperli D., Rosenberg M.;
RT "Affecting gene expression by altering the length and sequence
RT of the 5' leader";
RL Proc. Natl. Acad. Sci. U.S.A. 81:7698-7702(1984).
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RN [6]
RC pYSK9-12 from yeast galK gene
RA Clark B.F.C.;
RT ;
RL Unpublished (1985).
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CC Vector pDSP1 contains two independent mammalian transcription
CC cassettes containing SV40 early gene regulatory elements
CC controlling either the E. coli galK or xgprt genes.
CC The regulatory elements of the galK cassette are
CC bounded by multiple restriction sites allowing for easy removal and
CC replacement of the regulatory sequences or the galK gene itself.
CC NCBI gi: 208227
CC NM (pDSP1 seg 1)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP ()
CC HO (E.coli)
CC CP ()
CC FN (promoter analysis)(expression eukaryote)
CC SE (color red/white)
CC PA (pSVK3gpt from pSVKgpt)(pKSVgpt*N from pMLRg*s)
CC BR (pDSP1 seg 2)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
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FT misc_feature 0..0
FT /note="1. yeast galK gene
FT -> pYSK9-12
FT 1. E. coli galK gene
FT 2. E. coli gpt gene
FT -> pSVK
FT 1. pSVK
FT 2. xgprt gene BamHI-BamHI
FT -> pSVKgpt
FT 1. pSVKgpt remove HindIII-BglII 116bp, xgprt 5' leader
FT -> pSVKgpt*
FT 1. pSVKgpt remove HindIII-MluI, SV40 and galK gene
FT 2. linker HindIII-blunt end 6bp aagctt
FT 3. pYSK9-12 EcoRI-MluI, galK gene
FT mung bean nuclease:
FT -> pSVK3gpt
FT 1. pSVK NdeI, in pBR322 2299
FT fill in
FT 2. pSVKgpt* BamHI-BamHI, xgprt gene
FT fill in:fill in
FT -> plasmid
FT 1. plasmid remove HpaI-HpaI, SV40 t splice
FT HindIII linker 6bp aagctt
FT -> plasmid2
FT 1. plasmid2 PvuII, SV40 early promoter
FT 2. M13 34bp, MCS
FT -> pMLRg*s
FT 1. pMLRg*s HindIII, SV40 polyA
FT fill in
FT MCS linker 34bp, M13 MCS
FT BamHI, xgprt gene
FT fill in
FT MCS linker 34bp, M13 MCS
FT -> plasmid3
FT 1. plasmid3 NcoI, SV40 early promoter
FT fill in
FT NcoI, SV40 early promoter
FT fill in
FT -> pKSVgpt*N
FT 1. pSVK3gpt SfiI-MluI
FT 2. pKSVgpt*N remove small SfiI-MluI
FT -> pDSP1 6993bp"
FT misc_recomb <1..>1
FT /note="pBR322 DNA end/M13 DNA start"
FT misc_recomb 38..39
FT /note="M13 DNA end/SV40 early promoter start"
FT promoter 39..384
FT /note="PRO SV40 early genes"
FT misc_recomb 384..385
FT /note="SV40 early promoter DNA end/synthetic linker
FT start"
FT CDS 395..1543
FT /note="GEN E. coli galactokinase gene (galK);
FT AA 5 is at 407"
FT misc_recomb 406..407
FT /note="synthetic linker end/E. coli galK cds start
FT (AA 5)"
FT misc_recomb 1589..1590
FT /note="E. coli galK DNA end/synthetic linker start"
FT misc_binding 1590..1641
FT /note="MCS"
FT misc_recomb 1641..1642
FT /note="synthetic linker end/SV40 DNA sart"
FT misc_recomb 1776..1777
FT /note="SV40 DNA end/synthetic linker start"
FT misc_binding 1777..1800
FT /note="MCS"
FT misc_recomb 1800..1801
FT /note="synthetic linker end/SV40 late region DNA
FT start"
FT CDS 395..1543
FT /note="GEN E. coli galactokinase gene (galK);
FT NCBI gi: 208230"
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SQ Sequence 2553 BP; 653 A; 666 C; 601 G; 633 T; 0 other;
agcttggctg caggtcgacg gatcgatccc cgggaattct gtggaatgtg tgtcagttag
ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
ctccgcccag ttccgcccat tctccgcccc atgcatggct gactaatttt ttttatttat
gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
ggaggcctag gcttttgcaa aaagcttggc caccatggct ctccaagaaa aaacacaatc
tctgtttgcc aacgcatttg gctaccctgc cactcacacc attcaggcgc ctggccgcgt
gaatttgatt ggtgaacaca ccgactacaa cgacggtttc gttctgccct gcgcgattga
ttatcaaacc gtgatcagtt gtgcaccacg cgatgaccgt aaagttcgcg tgatggcagc
cgattatgaa aatcagctcg acgagttttc cctcgatgcg cccattgtcg cacatgaaaa
ctatcaatgg gctaactacg ttcgtggcgt ggtgaaacat ctgcaactgc gtaacaacag
cttcggcggc gtggacatgg tgatcagcgg caatgtgccg cagggtgccg ggttaagttc
ttccgcttca ctggaagtcg cggtcggaac cgtattgcag cagctttatc atctgccgct
ggacggcgca caaatcgcgc ttaacggtca ggaagcagaa aaccagtttg taggctgtaa
ctgcgggatc atggatcagc taatttccgc gctcggcaag aaagatcatg ccttgctgat
cgattgccgc tcactgggga ccaaagcagt ttccatgccc aaaggtgtgg ctgtcgtcat
catcaacagt aacttcaaac gtaccctggt tggcagcgaa tacaacaccc gtcgtgaaca
gtgcgaaacc ggtgcgcgtt tcttccagca gccagccctg cgtgatgtca ccattgaaga
gttcaacgct gttgcgcatg aactggaccc gatcgtggca aaacgcgtgc gtcatatact
gactgaaaac gcccgcaccg ttgaagctgc cagcgcgctg gagcaaggcg acctgaaacg
tatgggcgag ttgatggcgg agtctcatgc ctctatgcgc gatgatttcg aaatcaccgt
gccgcaaatt gacactctgg tagaaatcgt caaagctgtg attggcgaca aaggtggcgt
acgcatgacc ggcggcggat ttggcggctg tatcgtcgcg ctgatcccgg aagagctggt
gcctgccgta cagcaagctg tcgctgaaca atatgaagca aaaacaggta ttaaagagac
tttttacgtt tgtaaaccat cacaaggagc aggacagtgc tgaacgaaac tcccgcactg
gcacccgatg gtcagccgta ccgactgttc aagctagaat tctctagagc tcgctgatca
gcctcgaggc cagctagctt gaacttgttt attgcagctt ataatggtta caaataaagc
aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
tccaaactca tcaatgtatc ttatcatgtc tggatccctc gaggggggat cccgtcgacc
gatccccagg aagctcctct gtgtcctcat aaaccctaac ctcctctact tgagaggaca
ttccaatcat aggctgccca tccaccctct gtgtcctcct gttaattagg tcacttaaca
aaaaggaaat tgggtagggg tttttcacag accgctttct aagggtaatt ttaaaatatc
tgggaagtcc cttccactgc tgtgttccag aagtgttggt aaacagccca caaatgtcaa
cagcagaaac atacaagctg tcagctttgc acaagggccc aacaccctgc tcatcaagaa
gcactgtggt tgctgtgtta gtaatgtgca aaacaggagg cacattttcc ccacctgtgt
aggttccaaa atatctagtg ttttcatttt tacttggatc aggaacccag cactccactg
gataagcatt atccttatcc aaaacagcct tgtggtcagt gttcatctgc tgactgtcaa
ctgtagcatt ttttggggtt acagtttgag caggatattt ggtcctgtag tttgctaaca
caccctgcag ctccaaaggt tccccaccaa cagcaaaaaa atgaaaattt gacccttgaa
tgggttttcc agcaccattt tcatgagttt tttgtgtccc tgaatgcaag tttaacatag
cagttacccc aataacctca gttttaacag taacagcttc ccacatcaaa atatttccac
aggttaagtc ctcatttaaa ttaggcaaag gaa
//