back Return to this vector's summary.
ID   PDSP12     preliminary; circular DNA; SYN; 6993 BP.
XX
AC   K03394;
XX
DT   18-NOV-1986 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pDSP1 seg 2 - incomplete, segment 2, xgprt gen.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pSVKgpt from pSVK
RC   pSVKgpt* from pSVKgpt
RC   pSVK3gpt from pSVKgpt & pYSK9-12 & linker
RC   pMLRg*s from pSVK & pSVKgpt*
RC   pKSVgpt*N- from pMLRg*s
RC   pDSP1 from pKSVgpt*N- & pSVK3gpt
RA   Pfarr D.S., Sathe G., Reff M.E.;
RT   "Laboratory methods: A highly modular cloning vector for the
RT   analysis of eukaryotic genes and gene regulatory elements";
RL   DNA 4:461-467(1985).
XX
RN   [2]
RP   1-2143
RC   pDSP1
RA   Reff M.E.;
RT   ;
RL   Submitted (29-MAY-1986) to GenBank on computer-readable media by:
RL   Reff M.E., Smith Kline and French Labs, Swedeland PA 19479.
XX
CC   Vector pDSP1 contains two independent mammalian transcription
CC   cassettes containing SV40 early gene regulatory elements
CC   controlling either the E.coli galK or xgprt genes.
CC   The regulatory elements of the galK cassette are
CC   bounded by multiple restriction sites allowing for easy removal and
CC   replacement of the regulatory sequences or the galK gene itself.
CC   NCBI gi: 208228
CC   NM (pDSP1 seg 2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (promoter analysis)
CC   SE (color red/white)
CC   PA (SV40)(E.coli)
CC   BR (pDSP1 seg 1)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
XX
FT   misc_feature    0..0
FT                   /note="1. yeast galK gene
FT                   -> pYSK9-12
FT                   1. pSVK
FT                   2. xgprt gene BamHI-BamHI
FT                   -> pSVKgpt
FT                   1. pSVKgpt remove HindIII-BglII 116bp, xgprt 5' leader
FT                   -> pSVKgpt*
FT                   1. pSVKgpt remove HindIII-MluI, SV40 and galK gene
FT                   2. linker HindIII-blunt end 6bp aagctt
FT                   3. pYSK9-12 EcoRI-MluI, galK gene
FT                   mung bean nuclease:
FT                   -> pSVK3gpt
FT                   1. pSVK NdeI, in pBR322 2299
FT                   fill in
FT                   2. pSVKgpt* BamHI-BamHI, xgprt gene
FT                   fill in:fill in
FT                   -> plasmid
FT                   1. plasmid remove HpaI-HpaI, SV40 t splice
FT                   2. HindIII linker 6bp aagctt
FT                   -> plasmid2
FT                   1. plasmid2 PvuII, SV40 early promoter
FT                   2. M13 34bp, MCS
FT                   -> pMLRg*s
FT                   1. pMLRg*s HindIII, SV40 polyA
FT                   fill in
FT                   MCS linker 34bp, M13 MCS
FT                   BamHI
FT                   fill in
FT                   MCS linker 34bp, M13 MCS
FT                   -> plasmid3
FT                   1. plasmid3 NcoI, SV40 early promoter
FT                   fill in
FT                   NcoI, SV40 early promoter
FT                   fill in
FT                   -> pKSVgpt*N
FT                   1. pSVK3gpt SfiI-MluI
FT                   2. pKSVgpt*N remove small SfiI-MluI
FT                   -> pDSP1 6993bp"
FT   misc_recomb     <1..>1
FT                   /note="pBR322 DNA end/SV40 early DNA start"
FT   promoter        1..355
FT                   /note="PRO SV40 early genes"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   misc_recomb     355..356
FT                   /note="SV40 early DNA end/E. coli xgprt gene start"
FT   misc_recomb     1312..1313
FT                   /note="E. coli xgprt gene end/SV40 DNA start"
FT   misc_recomb     1904..1905
FT                   /note="SV40 DNA end/SV40 poly-A early DNA start"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   CDS             434..892
FT                   /note="GEN E. coli xanthine phosphoribosyltransferase
FT                   gene (xpgrt); NCBI gi: 208231"
XX
SQ   Sequence 2143 BP; 576 A; 495 C; 473 G; 599 T; 0 other;
     gatccggctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     tgcatggctg actaattttt tttatttatg cagaggccga ggccgcctcg gcctctgagc
     tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctgatct
     ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     ccgtggcggt ctggtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
     acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttctag
     taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
     ttgtgatgag cgatgccgaa gctaccgacg atgatttata cgatacggtg attggctacc
     gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
     ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
     agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
     gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
     ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
     cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     tggtttgtcc aaactcatca atgtatctta tcatgtctgg atc
//