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ID   PE194COP6  preliminary; circular DNA; SYN; 3728 BP.
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AC   ATCC39089;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Bacillus/Staphylococcus plasmid vector pE194 cop6 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pE194 cop-6 or pBD15 from pE194
RC   pE194 tyl-6 from pE194
RA   Weisblum B., Iordanescu B.;
RT   ;
RL   Unpublished (1983).
RL   U.S. Patent No. 4,419,450 dated Dec. 6, 1983.
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RN   [2]
RC   pE194 from S. aureus
RC   pE194 tyl-1 from pE194
RC   pE194 cop-6 or pBD15 from pE194
RC   pE194 tyl-6 from pE194
RA   Weisblum B., Graham M.Y., Gryczan T.J., Dubnau D.;
RT   "Plasmid copy number control: isolation and characterization of
RT   high-copy-number mutants of plasmid pE194";
RL   J. Bacteriol. 137:635-643(1979).
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CC   This was used in the construction of cloning vector pOS4 (ATCC 39097).
CC   Temperature sensitive.
CC   For full induction, the plasmid requires a period of induction by
CC   subinhibitory concentrations of erythromycin. [2]
CC   Plasmid is a high copy mutant of pE194, a natural isolate from
CC   Staphylococcus aureus that confers resistance to macrolide,
CC   lincosamide & streptogramin type B (MLS) antibiotics. [2]
CC   CPC International <-- Bacillus Genetic Stock Center <-- B. Weisblum.
CC   (ATCC staff)
CC   Medium is 18 trypticase soy agar.
CC   NM (pE194 cop6)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (Bacillus subtilis BSV IE7)(Bacillus)(Staphylococcus aureus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pE194)
CC   BR ()
CC   OF (pOS4)
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pE194 3728bp
FT                   mutagenesis
FT                   -> pE194 cop-6 3728bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BclI-AccI-PstI"
FT   rep_origin      0..0
FT                   /note="ORI Staphylococcus pE194"
FT   CDS             0..0
FT                   /note="ANT Staphylococcus erythromycin resistance gene
FT                   (ery)"
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SQ   Sequence 3728 BP; 1362 A; 460 C; 722 G; 1184 T; 0 other;
     gatcagtaca agaaagatac tgtatttcat aaacaggaac tgcaagaagt taaggatgag
     ttacagaagg caaataagca gttacagagt ggaatagagc atatgaggtc tacgaaaccc
     tttgattatg aaaatgagcg tacaggtttg ttctctggac gtgaagagac tggtagaaag
     atattaactg ctgatgaatt tgaacgcctg caagaaacaa tctcttctgc agaacggatt
     gttgatgatt acgaaaatat taagagcaca gactattaca cagaaaatca agaattaaaa
     aaacgtagag agagtttgaa agaagtagtg aatacatgga aagaggggta tcacgaaaaa
     agtaaagagg ttaataaatt aaagcgagag aatgatagtt tgaatgagca gttgaatgta
     tcagagaaat ttcaagctag tacagtgact ttatatcgtg ctgcgagggc gaatttccct
     gggtttgaga aagggtttaa taggcttaaa gagaaattct ttaatgattc caaatttgag
     cgtgtgggac agtttatgga tgttgtacag gataatgtcc agaaggtcga tagaaagcgt
     gagaaacagc gtacagacga tttagagatg tagaggtact tttatgccga gaaaactttt
     tgcgtgtgac agtccttaaa atatacttag agcgtaagcg aaagtagtag cgacagctat
     taactttcgg ttgcaaagct ctaggatttt taatggacgc agcgcatcac acgcaaaaag
     gaaattggaa taaatgcgaa atttgagatg ttaattaaag acctttttga ggtctttttt
     tcttagattt ttggggttat ttaggggaga aaacataggg gggtactacg acctcccccc
     taggtgtcca ttgtccattg tccaaacaaa taaataaata ttgggttttt aatgttaaaa
     ggttgttttt tatgttaaag tgaaaaaaac agatgttggg aggtacagtg atggttgtag
     atagaaaaga agagaaaaaa gttgctgtta ctttaagact tacaacagaa gaaaatgaga
     tattaaatag aatcaaagaa aaatataata ttagcaaatc agatgcaacc ggtattctaa
     taaaaaaata tgcaaaggag gaatacggtg cattttaaac aaaaaaagat agacagcact
     ggcatgctgc ctatctatga ctaaattttg ttaagtgtat tagcaccgtt attatatcat
     gagcgaaaat gtaataaaag aaactgaaaa caagaaaaat tcaagaggac gtaattggac
     atttgtttta tatccagaat cagcaaaagc cgagtggtta gagtatttaa aagagttaca
     cattcaattt gtagtgtctc cattacatga tagggatact gatacagaag gtaggatgaa
     aaaagagcat tatcatattc tagtgatgta tgagggtaat aaatcttatg aacagataaa
     aataattaac agaagaattg aatgcgacta ttccgcagat tgcaggaagt gtgaaaggtc
     ttgtgagata tatgcttcac atggacgatc ctaataaatt taaatatcaa aaagaagata
     tgatagttta tggcggtgta gatgttgatg aattattaaa gaaaacaaca acagatagat
     ataaattaat taaagaaatg attgagttta ttgatgaaca aggaatcgta gaatttaaga
     gtttaatgga ttatgcaatg aagtttaaat ttgatgattg gttcccgctt ttatgtgata
     actcggcgta tgttattcaa gaatatataa aatcaaatcg gtataaatct gaccgataga
     ttttgaattt aggtgtcaca agacactctt ttttcgcacc agcgaaaact ggtttaagcc
     gactgcgcaa aagacataat cgattcacaa aaaataggca cacgaaaaac aagttaaggg
     atgcagttta tgcatccctt aacttactta ttaaataatt tatagctatt gaaaagagat
     aagaattgtt caaagctaat attgtttaaa tcgtcaattc ctgcatgttt taaggaattg
     ttaaattgat tttttgtaaa tattttcttg tattctttgt taacccattt cataacgaaa
     taattatact tttgtttatc tttgtgtgat attcttgatt tttttctact taatctgata
     agtgagctat tcactttagg tttaggatga aaatattctc ttggaaccat acttaatata
     gaaatatcaa cttctgccat taaaagtaat gccaatgagc gttttgtatt taataatctt
     ttagcaaacc cgtattccac gattaaataa atctcattag ctatactatc aaaaacaatt
     ttgcgtatta tatccgtact tatgttataa ggtatattac catatatttt ataggattgg
     tttttaggaa atttaaactg caatatatcc ttgtttaaaa cttggaaatt atcgtgatca
     acaagtttat tttctgtagt tttgcataat ttatggtcta tttcaatggc agttacgaaa
     ttacacctct ttactaattc aagggtaaaa tggccttttc ctgagccgat ttcaaagata
     ttatcatgtt catttaatct tatatttgtc attattttat ctatattatg ttttgaagta
     ataaagtttt gactgtgttt tatatttttc tcgttcatta taaccctctt taatttggtt
     atatgaattt tgcttattaa cgattcatta taaccactta ttttttgttt ggttgataat
     gaactgtgct gattacaaaa atactaaaaa tgcccatatt ttttcctcct tataaaatta
     gtataattat agcacgagct ctgataaata tgaacatgat gagtgatcgt taaatttata
     ctgcaatcgg atgcgattat tgaataaaag atatgagaga tttatctaat ttcttttttc
     ttgtaaaaaa agaaagttct taaaggtttt atagttttgg tcgtagagca cacggtttaa
     cgacttaatt acgaagtaaa taagtctagt gtgttagact ttatgaaatc tatatacgtt
     tatatatatt tattatccgg aggtgtagca tgtctcattc aattttgagg gttgccagag
     ttaaaggatc aagtaataca aacgggatac aaagacataa tcaaagagag aataaaaact
     ataataataa agacataaat catgaggaaa catataaaaa ttatgatttg attaacgcac
     aaaatataaa gtataaagat aaaattgatg aaacgattga tgagaattat tcagggaaac
     gtaaaattcg gtcagatgca attcgacatg tggacggact ggttacaagt gataaagatt
     tctttgatga tttaagcgga gaagaaatag aacgattttt taaagatagc ttggagtttc
     tagaaaatga atacggtaag gaaaatatgc tgtatgcgac tgtccatctg gatgaaagag
     tcccacatat gcactttggt tttgtccctt taacagagga cgggagattg tctgcaaaag
     aacagttagg caacaagaaa gactttactc aattacaaga tagatttaat gagtatgtga
     atgagaaagg ttatgaactt gaaagaggca cgtccaaaga ggttacagaa cgagaacata
     aagcgatg
//