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ID   PE8UCAT    preliminary; circular DNA; SYN; 7000 BP.
AC   ATCC37764;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Vertebrate/E.coli plasmid vector pE8UCAT - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pEMBL8-CAT from pEMBL8 & cat gene
RC   pUMSSB1 from mouse c-mos gene
RC   pE8UCAT from pEMBL8-CAT & pUMSSB1
RA   Piaggio G.;
RT   ;
RL   Focus 12:83-84(1990).
CC   This is a promoter cloning vector suitable for studying
CC   transcriptional activity of very weakly active promoter fragments.
CC   This was constructed by inserting a 1.025 kb SalI fragment from
CC   pUMSSB1 containing the strong transcription terminator from the mouse
CC   c-mos gene (UMS = upstream mouse sequence) into the EcoRI site of
CC   pEMBL8-CAT using EcoRI linkers.
CC   The presence of the terminator upstream of the cloning sites of the
CC   polylinker prevents transcription that starts at cryptic promoters in
CC   the vector sequence.
CC   Restriction digests of the clone give the following sizes (kb):
CC   BamHI--6.6; EcoRI--5.4, 1.1, 0.4; HindIII--6.6. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli HB101)(vertebrate cells)(E.coli)(E.coli 71/18)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. mouse, c-mos gene
FT                   -> pUMSSB1
FT                   1. pEMBL8+ 3939bp, ori/amp gene
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   -> pEMBL8-CAT 4712bp
FT                   1. pEMBL8-CAT 4712bp, ori/amp gene/cat gene
FT                   2. pUMSSB1
FT                   -> pE8UCAT 7000bp"
FT   misc_binding    0..0
FT                   /note="MCS XmaI-SmaI-BamHI-HincII-AccI-SalI-PstI-
FT                   HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique XmaI-SmaI-BamHI-HincII-AccI-SalI-
FT                   PstI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml);
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;