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ID   PEFT1      preliminary; circular DNA; SYN; 13000 BP.
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AC   IG1161;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pEFT1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pON3 from pBR322 & pON1
RC   pON5 from pON3 & lambda
RC   pON163 from pON5 & ARS1
RC   pDW222, pDW225 from HIS2 gene & URA gene
RC   pDW227 from pGEM-3 & pON163
RC   pDW229 from pGEM-3 & pON163
RC   pDW232 from pGEM-3 & pDW227 & pDW229
RA   Weilguny D., Praetorius M., Carr A.M., Egel R., Nielsen O.;
RT   "New vectors in fission yeast: application for cloning the his2
RT   gene";
RL   Gene 99:47-54(1991).
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CC   NM (pEFT1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Invitrogen)
CC   HO (S.cerevisiae)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. YEp24 HindIII-HindIII 1166bp 2273..3439, URA3 gene
FT                   3. yeast 2 micron, ori
FT                   4. yeast, EFT1 gene
FT                   -> pEFT1 13000bp"
FT   promoter        0..0
FT                   /note="PRO yeast EFT1 gene"
FT   misc_binding    0..0
FT                   /note="MCS SalI-PstI-SphI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(0..0)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             complement(0..0)
FT                   /note="ANT yeast URA3 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
//