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ID   PEGL3      preliminary; circular DNA; SYN; 1 BP.
AC   IG5120;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector pEGL3 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pEGL117 from pVAL-1 & pHZ117
RC   pHZ117delta from pUC19 & pHZ117
RC   pEGL1 from pVAL-1 & pHZ117delta
RC   pKS100 from pJS139 & pBluescript KS
RC   pUCp1 from pUC19 & pKS100
RC   pUCpdelta from pUCp1
RC   pEGL3 from pEGL1 & pUCpdelta
RC   pEGL106 from pEGL1 & pUCpdelta
RA   Abratt V.R., Zappe H., Woods D.R.;
RT   "A reporter gene vector to investigate the regulation of glutamine
RT   synthetase in Bacteroides fragilis Bf1";
RL   J. Gen. Microbiol. 139:59-65(1993).
RN   [2]
RC   pHZ100 from pUC19 & C.acetobutylicum eglA gene
RC   [pHZ117 from pUC19 & C.acetobutylicum eglA gene]
RA   Zappe H., Jones W.A., Jones D.T., Woods D.R.;
RT   "Structure of an endo-beta-1,4-glucanase gene from Clostridium
RT   acetobutylicum P262 showing homology with endoglucanase genes
RT   from Bacillus species";
RL   Appl. Environ. Microbiol. 54:1289-1292(1988).
RN   [3]
RC   pJS139 from pEcoR251 & Bacteroides fragilis glnA gene
RC   pJS139.1, pJS139.2 from pJS139
RA   Southern J.A., Parker J.R., Woods D.R.;
RT   "Expression and purification of glutamine synthetase cloned from
RT   Bacteroides fragilis";
RL   J. Gen. Microbiol. 132:2827-2835(1986).
RN   [4]
RC   pTB1 from pBR328 & Tn4351, Bacteroides ery gene/Cc gene
RC   pVAL-1 from pTB1 & pB8-51
RA   Valentine P.J., Shoemaker N.B., Salyers A.A.;
RT   "Mobilization of Bacteroides plasmids by Bacteroides conjugal
RT   elements";
RL   J. Bacteriol. 170:1319-1324(1988).
RN   [5]
RC   pB8-51 from Bacteroides
RA   Mays T.D.;
RT   ;
RL   Thesis [Virginia Polytechnic Inst.] 0:0-0(1978).
RN   [6]
RC   pDP1 from pB8-51
RC   pCG21 from pB8-51
RC   pEG-3 from pB8-51
RC   pEG920 from pB8-51
RA   Shoemaker N.B., Getty C.E., Guthrie E.P., Salyers A.A.;
RT   "Regions in Bacteroides plasmids pBFTM10 and pB8-51 that allow
RT   Escherichia coli-Bacteroides shuttle vectors to be mobilized by
RT   IncP plasmids and by a conjugative Bacteroides tetracycline
RT   resistance element";
RL   J. Bacteriol. 166:959-965(1986).
RN   [7]
RC   pEcoR251 from pBR322 & E.coli EcoRI gene & lambda, pR
RA   Zabeau M.M.;
RT   ;
RL   Unpublished (1986).
CC   fusion region. not original sequence.
CC   NM (pEGL3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pHZ117 from pUC19)(pVAL-1)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. Bacteroides
FT                   -> pB8-51 4200bp
FT                   1. pBR328 EcoRI
FT                   2. Tn4351 EcoRI-EcoRI, Bacteroides ery gene/Cc gene
FT                   -> pTB1 8700bp
FT                   1. pTB1 large ClaI-ClaI, tet/amp/ery genes
FT                   2. pB8-51 TaqI
FT                   -> pVAL-1 11000bp
FT                   1. pUC19
FT                   2. C.acetobutylicum, eglA gene
FT                   -> pHZ117 4400bp
FT                   1. pUC19 remove SmaI-PstI, MCS
FT                   2. pHZ117 SspI-PstI, eglA gene
FT                   -> pHZ117delta 4000bp
FT                   1. pVAL-1 remove small EcoRI-PstI
FT                   2. pHZ117delta EcoRI-PstI, eglA gene
FT                   -> pEGL1 10500bp
FT                   1. pBR322 [from pCL series]
FT                   2. lambda, pR [from pCL series]
FT                   3. E.coli EcoRI gene [unique BglII]
FT                   -> pEcoR251
FT                   1. pEcoR251 BglII, in EcoRI gene
FT                   2. Bacteroides fragilis Sau3AI-Sau3AI 8000bp, glnA
FT                   -> pJS139
FT                   1. pJS139 XhoI-XhoI 2400bp, glnA promoter/gene
FT                   2. pBluescript KS SalI
FT                   -> pKS100 5500bp
FT                   1. pUC19 remove EcoRI-SmaI, MCS
FT                   2. pKS100 EcoRI-SmaI 1250bp, glnA promoter/gene
FT                   -> pUCp1 3800bp
FT                   1. pUCp1 PstI
FT                   exonuclease III
FT                   -> pUCpdelta series
FT                   1. pEGL1 EcoRI-KpnI
FT                   :blunt end
FT                   2. pUCpdelta series EcoRI-SphI 508bp, glnA -108/XhoI
FT                   :blunt end
FT                   -> pEGL3"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;