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ID   PEHK255    preliminary; circular DNA; SYN; 1 BP.
AC   IG5179;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pEHK255 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pEHK11 from pWW53-4 & pUC19
RC   pEHK13 from pEHK11
RC   pEHK15 from pEHK13
RC   pEHK255 from pEHK15
RC   pEHK355 from pEHK255
RC   pEHK455 from pEHK355 & pKT230
RA   Keil S., Keil H.;
RT   "Construction of a cassette enabling regulated gene expression in the
RT   presence of aromatic hydrocarbons";
RL   Plasmid 27:191-199(1992).
RN   [2]
RC   pWW53 from Pseudomonas putida, TOL plasmid
RC   pWW53-4 from RP4 & pWW53
RC   pWW53-3301, pWW53-3311 from pKT230 & pWW53
RA   Keil S., Keil H., Pickup R.W., Williams P.A.;
RT   "Evolutionary conservation of genes coding for meta pathway enzymes
RT   within TOL plasmids pWWO and pWW53";
RL   J. Bacteriol. 164:887-895(1985).
RN   [3]
RC   pNJ5000 from pRP1
RC   pNJ5070 from pNJ5000 & Tn7
RC   [lambda 317c from lambda & trpE gene]
RC   pNJ5073 from pNJ5070 & lambda 317c, trpE gene
RC   pNJ927 from pTB92 & pRP1
RC   pNJ9279 from pNJ927
RA   Grinter N.J.;
RT   "A broad host range cloning vector transposable to various replicons";
RL   Gene 21:133-143(1983).
RN   [4]
RC   pRP1 from RP4 & Tn7
RA   Barth P.T., Grinter N.J.;
RT   "Map of plasmid RP4 derived by insertion of transposon C";
RL   J. Mol. Biol. 113:455-474(1977).
RN   [5]
RC   pGSS15 from E. coli IncQ group
RA   Sharpe G.;
RT   ;
RL   Unpublished (1983).
CC   pWWO is a TOL plasmid.
CC   MCS. promoter. RBS. not original sequence.
CC   NM (pEHK255)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pWW53-4 from pWWO)(pKT230)(pUC19)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. Pseudomonas putida, TOL plasmid
FT                   -> pWW53 105000bp
FT                   1. pWW53 35000bp, tol/xylR
FT                   2. RP4 56000bp
FT                   -> pWW53-4 91000bp [kan/tet/tol/xylR]
FT                   1. pWW53-4 EcoRI-EcoRI 17500bp, tol/xylR
FT                   2. pUC19 EcoRI 2686bp 397..397
FT                   -> pEHK11 20400bp [lac promoter]
FT                   1. pEHK11 remove small SacI-SacI
FT                   -> pEHK13
FT                   1. pEHK13 remove small KpnI-SacI
FT                   remove small BamHI-BglII
FT                   -> pEHK15 [xylR/upper pathway ORF]
FT                   1. pEHK15 BamHI
FT                   BAL31 nuclease, remove 500bp/BstEII between 2 operons
FT                   -> pEHK255"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;