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ID   PEJL428    preliminary; circular DNA; SYN; 71 BP.
AC   M33420;
DT   12-JUN-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pEJL428 - incomplete, MCS & promoter.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-71
RC   pEJL408 from pBR322 & pJFF350
RC   pEJL426 from pBR327 & oligo
RC   pEJL427 from pEJL426 & pEJL408
RC   pEJL428 from pEJL426 & pJFF350
RC   pEJL432 from pEJL426 & phage Mu dII
RC   pEJL435 from pEJL427 & pEJL432
RC   pEJL430 from pEJL428 & cat gene
RC   pEJL449 from pEJL408 & pEJL430
RA   Joseph-Liauzun E., Fellay R., Chandler M.S.;
RT   "Transposable elements for efficient manipulation of a wide range of
RT   gram-negative bacteria: Promoter probes and vectors for foreign
RT   genes";
RL   Gene 85:83-89(1989).
RN   [2]
RC   pJFF98 from pMP3 & pHP45omega-Km & pSUP202Tn5, RP4 oriT
RC   [pGB2 from pGB series]
RC   [pGB2A from pGB2]
RC   pJFF348 from pJFF98 & pGB2A
RC   pJFF349 from pJFF348 & pBR322
RC   pUC7IS1oriT from pUC7 & pMJ4 & RP4
RC   pJFF350 from pJFF98 & pUC7IS1oriT
RA   Fellay R., Krisch H.M., Prentki P., Frey J.;
RT   "Omegon-Km: a transposable element designed for in vivo insertional
RT   mutagenesis and cloning of genes in Gram-negative bacteria";
RL   Gene 76:215-226(1989).
RN   [3]
RC   pMJ4 from IS1
RA   Jakowec M., Prentki P., Chandler M., Galas D.;
RT   "Mutational analysis of the open reading frames in the transposable
RT   element IS1";
RL   Genetics 120:47-55(1988).
RN   [4]
RC   pMP3 from pBR322 & IS1 & omega transposon
RA   Prentki P., Pham M.H., Gamas P., Chandler M., Galas D.J.;
RT   "Artificial transposable elements in the study of the ends of IS1";
RL   Gene 61:91-101(1987).
CC   from Figure 3 of [1].
CC   From EMBL 27   entry AG420; dated 26-MAY-1990.
CC   NCBI gi: 207833
CC   NM (pEJL428)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(promoter analysis)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. omega transposon, IS1 28bp-spec/strep-IS1 28bp
FT                   \ #M60473
FT                   -> pMP3
FT                   1. pMP3 remove BamHI-BamHI 2000bp
FT                   2. pHP45omega-Km BamHI-BamHI 2200bp, spec/kan gene
FT                   -> plasmid
FT                   1. pACYC184 4245bp
FT                   2. RP4, oriT
FT                   -> pSUP101
FT                   1. pBR325
FT                   2. pSUP101
FT                   -> pSUP202-1
FT                   1. pSUP202-1
FT                   2. Tn5 BglII-BamHI 1541bp 1516..3057, kan/neo gene
FT                   -> pSUP202Tn5
FT                   1. plasmid remove EcoRI-EcoRV 189bp,
FT                   \ pBR322 4360..4361..188/7000bp
FT                   2. pSUP202Tn5 EcoRI-EcoRV 3000bp, RP4 oriT
FT                   -> pJFF98 10000bp
FT                   1. IS1*, [not transposable]
FT                   -> pMJ4
FT                   1. pUC7 EcoRI 2674bp 231..231, may be 273..273
FT                   2. pMJ4 EcoRI-SalI 770bp, IS1* [not transposable]
FT                   3. RP4 HaeII-HaeII 760bp, RP4 oriT
FT                   -> pUC7IS1oriT 4200bp
FT                   1. pBR322
FT                   -> pGB2
FT                   1. pGB2
FT                   -> pGB2A
FT                   1. pJFF98 EcoRI-EcoRI, spec/kan gene
FT                   2. pGB2A EcoRI
FT                   -> pJFF348
FT                   1. pJFF348 SmaI
FT                   2. pBR322 PvuII-BglI 1422bp 2067..3489, ori
FT                   Klenow:Klenow
FT                   -> pJFF349
FT                   1. pJFF349 EcoRI
FT                   2. pUC7IS1oriT EcoRI-EcoRI, IS1*/RP4 oriT
FT                   -> pJFF350 5300bp
FT                   1. pBR322 EcoRI-PvuII 2068bp 4360..4361..2067, tet
FT                   Klenow:Klenow
FT                   2. pJFF350 EcoRI 5300bp
FT                   Klenow:Klenow
FT                   -> pEJL408 7400bp
FT                   1. pBR327 remove DraI-EcoRI 1127bp 2145..3272,
FT                   \ amp gene/2146bp
FT                   2. oligo EcoRI-IS1right-XhoI-XbaI-NdeI-ScaI-DraI 28bp
FT                   -> pEJL426 2174bp
FT                   1. pEJL426 EcoRI-FspI 1359bp 3272..3273..1358
FT                   2. pEJL408 DraI-BglII, kan gene
FT                   3. pJFF350 BglII-EcoRI, IS1/oriT/tet gene/IS1/IRL/kan
FT                   -> pEJL428"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 71 BP; 20 A; 15 C; 13 G; 23 T; 0 other;
     aattcggtaa tgactccaac ttattgatag agtactcata tgtctagact cgaggatccc
     gtcgttttac a