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ID   PEM180     preliminary; circular DNA; SYN; 1 BP.
AC   IG5140;
DT   01-OCT-1993 (Rel. 7, Created)
DT   01-APR-1995 (Rel. 11, Last updated, Version 1)
DE   E. coli plasmid vector pEM180 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pEM180, pEM182, pEM183 from pCM959
RC   pEM83 from pEM180 & pBR322
RC   pEM84 from pEM182 & pBR322
RC   pEM76 from pEM183 & pBR322
RC   pEM205, pEM206 from pCM959 & kan gene
RC   pEM212 from pEM205 & pEM83
RC   pEM216 from pEM205 & pEM84
RC   pEM221 from pEM205 & pEM76
RC   pEM102, pEM322, pEM190, pEM222, pEM232 from pEM series
RA   Stuitje A.R., Meijer M.;
RT   "Isolation and characterization of plasmids carrying a partially
RT   defective Escherichia coli replication origin";
RL   Nucleic Acids Res. 11:8007-8018(1983).
RN   [2]
RC   lambda asn132 from lambda & E. coli oriC
RC   lambda asn3 from lambda & E. coli oriC
RC   pCM959 from lambda asn3, E. coli oriC
RC   pOC2 from lambda asn132, E. coli oriC
RC   pOC12 from pOC2
RC   pOC34 from pOC2
RA   Meijer M., Beck E., Hansen F.G., Bergmans H.E., Messer W.,
RA   Von Meyenburg K., Schaller H.;
RT   "Nucleotide sequence of the origin of replication of the
RT   Escherichia coli K-12 chromosome";
RL   Proc. Natl. Acad. Sci. U.S.A. 76:580-584(1979).
RN   [3]
RC   pOC42 from pBR322 & pCM959, minichromosome
RA   Morelli G., Buhk H.J., Fisseau C., Lother H., Yoshinaga K., Messer W.;
RT   "Promoters in the region of the E. coli replication origin";
RL   Mol. Gen. Genet. 184:255-259(1981).
RN   [4]
RC   pJF407 from F' factor
RC   pCM432 from pJF407, F' factor
RC   pCM440 from pJF407, F' factor
RC   pCM482 from pJF407, F' factor
RC   pCM488 from pJF407, F' factor
RA   Von Meyenburg K., Hansen F.G., Neilsen L.D., Riise E.;
RT   "Origin of replication, oriC, of the Escherichia coli chromosome
RT   of specialized transducing phages lambda asn";
RL   Mol. Gen. Genet. 158:101-109(1977).
CC   mutations in oriC.
C   NM (pEM180)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pCM959 from pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   2. E. coli, 70kD/oriC/16kD/17kD/asnA
FT                   circularization point
FT                   -> lambda asn3
FT                   1. lambda asn3, E. coli 70kD/oriC/16kD/17kD/asnA
FT                   -> pCM959 4000bp [no lambda]
FT                   1. pCM959
FT                   mutation
FT                   -> pEM180"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;