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ID   PEMBLEX2   preliminary; circular DNA; SYN; 4420 BP.
AC   IG8024;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli phasmid vector pEMBLex2 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pPLc245 from lambda, pL
RC   pEMBLex2 from pEMBL8- & pPLc245, lambda pL
RC   pEMBLex3 from pEMBL8- & pCQV2, lambda cI857ts gene/pR
RC   pSP65ss from pSP65 & pD4
RC   plasmid from pBR322 & M13mp9
RC   pEX11 from plasmid & pSP65ss
RC   pEX18 from pEX11
RC   pEX21 from pEX18
RA   Sollazzo M., Frank R., Cesareni G.;
RT   "High-level expression of RNAs and proteins: the use of
RT   oligonucleotides for the precise fusion of coding-to-regulatory
RT   sequences";
RL   Gene 37:199-206(1985).
RN   [2]
RC   pcI857 from pACYC177 & lambda, cI857ts gene
RA   Zabeau M.;
RT   ;
RL   Unpublished (1985).
CC   NM (pEMBLex2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E. coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda HinfI-Sau3A/MboI 423bp 35365..35788,
FT                   \ pL
FT                   -> pPLc245
FT                   1. pEMBL8- remove BamHI-HindIII 20bp 239..259,
FT                   \ MCS/3919bp
FT                   2. pPLc245 Sau3A/MboI-HindIII 420bp, lambda pL
FT                   -> plasmid 4340bp
FT                   1. plasmid XbaI 4340bp, lambda pL
FT                   S1 nuclease
FT                   -> pEMBLex2 4420bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;