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ID   PEMBLYI27  preliminary; circular DNA; SYN; 6210 BP.
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AC   X04996; X04995; ATCC37403;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/E.coli phagemid vector pEMBLYi27 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pEMBLYi21, pEMBLYi22 from pEMBL9 & YEp24
RC   YEp24-H from YEp24
RC   pEMBLYe23, pEMBLYe24 from pEMBL9 & YEp24
RC   pEMBLYr25 from pEMBL9 & YRp7
RC   pEMBLYi27 from pEMBL9 & pCV13
RC   pEMBLYe30 from pEMBL9 & pCV13
RA   Baldari C., Cesareni G.;
RT   "Plasmids pEMBLY: new single-stranded shuttle vectors for the
RT   recovery and analysis of yeast DNA sequences";
RL   Gene 35:27-32(1985).
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CC   ssDNA-producing plasmid with polylinker in lacZ' and marker selectable
CC   in yeast.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pEMBLYi27)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (ATCC)
CC   HO (E.coli 71/18)(Saccharomyces cerevisiae)(E.coli)
CC   HO (E.coli JM series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. yeast 2 micron 6318bp
FT                   2. yeast 3400bp, LEU2 gene
FT                   -> pCV13 9700bp
FT                   1. pEMBL9+ ClaI 3939bp 1324..1324, near f1 ori
FT                   Klenow
FT                   2. pCV13 XhoI-SalI 2200bp,
FT                   \ yeast 2 micron/LEU2 gene [4774..8528]
FT                   \ yeast 2 micron SalI = none/SalI in LEU2 gene
FT                   \ yeast 2 micron XhoI = none/XhoI in LEU2 gene
FT                   Klenow:Klenow
FT                   -> pEMBLYi27 6200bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-AvaI-PstI-HindIII"
FT   misc_binding    0..0
FT                   /note="SIT unique BstEII-ClaI-EcoRV-KpnI-NarI-
FT                   BamHI-HindIII-PstI-SalI-SmaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage f1"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT yeast LEU2 gene"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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