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ID   PEP121     preliminary; circular DNA; SYN; 12500 BP.
AC   IG8026;
DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pEP121 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pHP3 from pBR313 & E.coli trp operon
RC   pHP12, pHP39 from pHP3 & linker & pBR345
RC   pEP121 from pHP12
RC   pEP392 from pHP39
RC   pEP3921 from pEP392
RC   pEP3923 from pEP392
RA   Enger-Valk B.E., Heyneker H.L., Oosterbaan R.A., Pouwels P.H.;
RT   "Construction of new cloning vehicles with genes of the tryptophan
RT   operon of Escherichia coli as genetic markers";
RL   Gene 9:69-85(1980).
RN   [2]
RC   pEP301 from pHP3
RC   pEP3012 from pEP301 & linker
RC   pEP3014 from pEP301 & linker
RC   pEP3015 from pEP301 & pHP3
RC   pEP292 from pHP39 & pEP3012
RC   pEP320 from pHP3 & pEP3015
RC   pEP702 from pHP3
RA   Enger-Valk B.E., Van Rotterdam J., Kos A., Pouwels P.H.;
RT   "Construction of new vectors for cloning of promoters";
RL   Gene 15:297-305(1981).
RN   [3]
RC   pHP16 from pHP3 & linker & pBR345
RC   pEP165 from pHP16 & pBR345
RC   pEP167 from pEP165
RC   pEP168 from pEP167 & linker
RC   pEP70 from pEP165 & linker
RC   pEP71 from pEP165 & linker
RC   pEP72 from pEP168 & linker
RC   pEP73 from pEP168 & linker
RC   pEP74 from pEP168 & linker
RC   pEP75 from pEP168 & linker
RC   pEP162 from pHP16
RC   pEP1651, pEP1652 from pEP162 & pEP165
RC   pEP200 from pEP168 & pEP1652 & linker
RC   pEP301 from pHP3
RC   pEP201 from pEP200 & pEP301
RC   pEP41 from pEP201
RC   pEP31 from pEP201 & linker
RC   pEP61 from pEP201 & pEP165
RC   pEP44 from pEP201 & linker & pEP73
RC   pEP2011 from pEP201
RC   pEP45 from pEP2011 & pEP72
RA   Enger-Valk B.E., Van Rotterdam J., Pouwels P.H.;
RT   "The construction of new vehicles for the cloning of transcription
RT   termination signals";
RL   Nucleic Acids Res. 9:1973-1989(1981).
CC   NM (pEP121)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pHP3 SmaI-SmaI 8700bp, trp operon
FT                   EcoRI linker 10bp ccgaattcgg:EcoRI linker 10bp
FT                   \ ccgaattcgg
FT                   EcoRI-EcoRI
FT                   2. pBR345 EcoRI 6300bp
FT                   -> pHP12 15000bp
FT                   1. pHP12 remove small HindIII-HindIII 2500bp, 12500bp
FT                   -> pEP121 12500bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;