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ID   PER3       preliminary; circular DNA; SYN; 1 BP.
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AC   IG8048;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pER3 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   p808-2 from p419-10
RC   p827-10 from p808-2 & pLBU3
RC   pER3, pER4 from p827-10
RC   pER3A, pER3B, pER3C, pER3D from pER3 & piVX
RC   plasmid from pER3B & pBR329
RC   from plasmid & pHC314
RA   Boros I., Lukascovich T., Baliko G., Venetianer P.;
RT   "Expression vectors based on the rac fusion promoter";
RL   Gene 42:97-100(1986).
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RN   [2]
RC   p419-10 from pBR322 & rrnB gene P2 promoter & T1/T2 terminators
RA   Boros I., Kiss A., Sain B., Somlyai G., Venetianer P.;
RT   "Cloning of the promoters of an Escherichia coli rRNA gene. New
RT   experimental system to study the regulation of rRNA transcription";
RL   Gene 22:191-201(1983).
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CC   NM (pER3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. E. coli, rrnB gene P2 promoter
FT                   3. E. coli, rrnB gene T1/T2 terminators
FT                   -> p419-10 5000bp
FT                   1. p419-10 StuI 5000bp, E. coli rrnB gene
FT                   EcoRI linker 6bp gaattc
FT                   -> p808-2 5000bp
FT                   1. p808-2 remove small EcoRI-HindIII, 16S rRNA gene
FT                   2. pLBU3 EcoRI-HindIII 415bp 2..417,
FT                   \ lacZalpha gene 5' [412bp]
FT                   -> p827-10 5000bp
FT                   1. p827-10 EcoRI 5000bp, lacZ/16S
FT                   BAL31 nuclease
FT                   -> pER3 5000bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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