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ID   PET01      preliminary; circular DNA; SYN; 1 BP.
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AC   IG1162; M68967;
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DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pET01 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pRSV-BC12-SEAP from RSV & SEAP & pBC12
RC   pL53In from pXf3 & pRSV-BC12-SEAP
RA   Auch D., Reth M.;
RT   "Exon trap cloning: using PCR to rapidly detect and clone exons
RT   from genomic DNA fragments";
RL   Nucleic Acids Res. 18:6743-6744(1990).
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RN   [2]
RC   [from none]
RA   Liu P., Legerski R., Siciliano J.;
RT   "Isolation of human transcribed sequences from human-rodent
RT   somatic cell hybrids";
RL   Science 246:813-815(1989).
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RN   [3]
RC   pETV-SD from DOL & ColE1 & alpha-gal/beta-gal
RA   Duyk G.M., Kim S., Myers R.M., Cox D.R.;
RT   "Exon trapping: a genetic screen to identify candidate transcribed
RT   sequences in cloned mammalian genomic DNA";
RL   Proc. Natl. Acad. Sci. U.S.A. 87:8995-8999(1990).
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RN   [4]
RC   plasmid from pBluescript series & pgTAT
RC   plasmid2 from plasmid & pSbeta-IVS2
RC   plasmid3 from plasmid2
RC   pSPL1 from plasmid3 & linker
RC   [pSPL3 from pSPL1]
RA   Buckler A.J., Chang D.D., Graw S.L., Brook J.D., Haber D.A.,
RA   Sharp P.A., Housman D.E.;
RT   "Exon amplification: A strategy to isolate mammalian genes based
RT   on RNA splicing";
RL   Proc. Natl. Acad. Sci. U.S.A. 88:4005-4009(1991).
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RN   [5]
RC   pDOP from pMSV-gpt & DO1
RA   Schwarzbauer J.E., Mulligan R.C., Hynes R.O.;
RT   "Efficient and stable expression of recombinant fibronectin
RT   polypeptides";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:754-758(1987).
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RN   [6]
RC   DO1 from pSV2neo & pZIP-SV(x)
RC   DO-MTIIgpt from pMSV-gpt & MTII gene promoter & pZIP-SV(x)
RC   DOL from pSV2neo & pZIP-SV(x) & MOV9-2 LTR & polyoma ori & MLV
RC   DOL- from DOL & DO1
RA   Korman A.J., Frantz J.D., Strominger J.L., Mulligan R.C.;
RT   "Expression of human class II major histocompatibility complex
RT   antigens using retrovirus vectors";
RL   Proc. Natl. Acad. Sci. U.S.A. 84:2150-2154(1987).
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RN   [7]
RC   pMOV-psi+ from MoMuLV & pBR322 & SV40
RC   pMOV-psi- from pMOV-psi+ & pSV2-gagpol
RC   pMSV-gpt from pBR322 & MSV & gpt gene & polyoma early region
RA   Mann R., Mulligan R.C., Baltimore D.;
RT   "Construction of a retrovirus packaging mutant and its use to
RT   produce helper-free defective retrovirus";
RL   Cell 33:153-159(1983).
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RN   [8]
RC   pPTHm127 from pPTHm122 & linker
RC   pMSV-hPTH from pPTHm127 & pMSV-gpt
RC   pMSV-hPTH-gpt from pMSV-hPTH & pL-10
RA   Hellerman J.G., Cone R.C., Potts J.T., Rich A., Mulligan R.C.,
RA   Kronenberg H.M.;
RT   "Secretion of human parathyroid hormone from rat pituitary cells
RT   infected with a recombinant retrovirus encoding preproparathyroid
RT   hormone";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:5340-5344(1984).
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RN   [9]
RC   plasmid from pBC12/CMV & HIV-1, tat gene/rev gene/env gene
RC   plasmid2 from pBC12/CMV & HIV-1 cDNA, tat gene/rev gene/env gene
RC   pgTAT from plasmid
RC   pgREV from plasmid
RC   pcTAT from plasmid
RC   pcREV from plasmid
RC   pgREVdeltaB from pgREV
RA   Malim M.H., Hauber J., Fenrick R., Cullen B.R.;
RT   "Immunodeficiency virus rev trans-activator modulates the
RT   expression of the viral regulatory genes";
RL   Nature 335:181-183(1988).
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RN   [10]
RC   pSbeta-NI from pSbeta-IVS2 & SVIV-Rabeta2, rabbit beta-globin gene
RC   ptk2.0-NI from pSbeta-NI & pSVOTKE, HSV tk gene & linkers
RC   ptk0.7, ptk1.1, ptkbetains from ptk2.0-NI
RA   Buchman A.R., Berg P.;
RT   "Comparison of intron-dependent and intron-independent gene
RT   expression";
RL   Mol. Cell. Biol. 8:4395-4405(1988).
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RN   [11]
RC   pSbeta-IVS2 from pSV2-gpt & SVIV-IVS2, rabbit beta-globin gene
RA   Buchman A.R., Fromm M., Berg P.;
RT   "Complex regulation of simian virus 40 early-region transcription
RT   from different overlapping promoters";
RL   Mol. Cell. Biol. 4:1900-1914(1984).
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RN   [12]
RC   SVIV-IVS2 from SV40 & rabbit beta-globin gene
RC   SVIV-Rabeta2 from SV40 & rabbit beta-globin gene
RA   Buchman A.R., Berg P.;
RT   "Unusual regulation of simian virus 40 early-region transcription
RT   in genomes containing two origins of DNA replication";
RL   Mol. Cell. Biol. 4:1915-1928(1984).
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RN   [13]
RC   pSVOTKE from HSV tk gene
RA   Fromm M.;
RT   ;
RL   Thesis [Stanford University] 0:0-0(1982).
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RN   [14]
RC   pPTHm122 from human preproparathyroid hormone gene
RA   Hendy G.N., Kronenberg H.M., Potts J.T., Rich A.;
RT   "Nucleotide sequence of cloned cDNAs encoding human
RT   preproparathyroid hormone";
RL   Proc. Natl. Acad. Sci. U.S.A. 78:7365-7369(1981).
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RN   [15]
RC   pDRalpha1 from DRalpha gene
RA   Wake C.T., Long E.O., Strubin M., Gross N., Accolla R.C., Carrel S.,
RA   Mach B.;
RT   "Isolation of cDNA clones encoding HLA-DR alpha chains";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:6979-6983(1982).
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RN   [16]
RC   pSV2-gagpol from pSV2 & MoMuLV gag pol region
RA   Mulligan R.C.;
RT   ;
RL   Unpublished (1983).
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CC   NM (pET01)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (USB)
CC   HO (E.coli)(COS cells)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   2. SV40 HindIII-PvuII 344bp 5172..5243..273,
FT                   \ early region
FT                   3. RSV, LTR
FT                   -> pET01"
FT   promoter        0..0
FT                   /note="PRO Rous sarcoma virus (RSV) long terminal
FT                   repeat (LTR)"
FT   CDS             0..0
FT                   /note="GEN E. coli phosphatase gene (nonfunctional)"
FT   exon            0..0
FT                   /note="E. coli phosphatase gene exon"
FT   intron          0..0
FT                   /note="E. coli phosphatase gene intron"
FT   misc_binding    0..0
FT                   /note="MCS"
FT   intron          0..0
FT                   /note="E. coli phosphatase gene intron"
FT   exon            0..0
FT                   /note="E. coli phosphatase gene exon"
FT   polyA_signal    0..0
FT                   /note="PLA SV40 early genes"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI SV40"
FT   promoter        0..0
FT                   /note="PRO SV40 early genes"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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