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ID   PET1       preliminary; circular DNA; SYN; 7700 BP.
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AC   ATCC39566;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pET1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   [pET1 from pBR322 & cat gene & kan gene]
RA   Efstratiadis A., Villa-Komaroff L.;
RT   "Cloning of double stranded cDNA";
RL   Genet. Eng. 1:15-36(1978).
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CC   A particularly versatile general purpose cloning vector.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pET1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli C600)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. Tn9 TaqI-TaqI 773bp 215..988, cat gene/#V00622
FT                   3. kan gene
FT                   -> pET1 7700bp"
FT   misc_binding    0..0
FT                   /note="SIT unique AvaI-BclI-EcoRI-PstI-XhoI-XmaI-
FT                   SalI-SphI-BamHI-EcoRV"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (cat); chloramphenicol resistance gene (cmr/cml)"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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