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ID PETHR1 preliminary; circular DNA; SYN; 15600 BP.
DT 01-JUL-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE Brevibacterium/Corynebacterium plasmid vector pEthr1 - incomplete.
KW cloning vector.
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
RC pGH2 from pBR322 & E.coli threonine operon
RC pEthr1 from pCG11 & pGH2
RL Unpublished (1987).
RL U.S. Patent No. 4,710,471 dated Dec. 1, 1987.
CC This was constructed by inserting a 8.8 kb BamHI fragment from pGH2
CC into the BglII site of pCG11 (ATCC 39022).
CC The original vials from the depositor are being used for distribution.
CC (ATCC staff)
CC pGH2 contains the threonine operon from E.coli. The BamHI
CC fragment of pGH2 in this construct encodes homoserine dehydrogenase.
CC Transformed strains do not require homoserine.
CC The construct contains the following restriction sites (in approximate
CC kb from the unique PstI site): EcoRI--1.1, 4.1, 13.1; XhoI--7.3;
CC Medium is 1236 LB plus kanamycin.
CC NM (pEthr1)
CC CM (no)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (ATCC)
CC HO (Corynebacterium glutamicum LA201)
CC HO (Brevibacterium)(Corynebacterium glutamicum)
CC CP ()
CC FN (cloning)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
FH Key Location/Qualifiers
FT misc_feature 0..0
FT /note="1. pCG1, ori/kan/spec
FT -> pCG2
FT 1. pCG2, ori/kan/spec
FT -> pCG4
FT 1. pCG4, ori/kan/spec
FT -> pCG11
FT 1. pCG11, ori/kan/spec
FT 2. pGH2
FT -> pEthr1 15600bp"
FT misc_binding 0..0
FT /note="SIT unique PstI-XhoI"
FT rep_origin 0..0
FT /note="ORI Corynebacterium pCG4"
FT CDS 0..0
FT /note="ANT Corynebacterium kanamycin resistance gene
FT CDS 0..0
FT /note="ANT Corynebacterium spectinomycin resistance
FT gene (spc)"
SQ Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;