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ID   PEW27      preliminary; circular DNA; SYN; 17299 BP.
AC   J01565; ATCC37124;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pEW27 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pEW27 from pBGP120
RC   pMM1 from pNOP42
RC   pEW2762 from pEW27
RC   pMM700 from pKO-1 & pNOP42
RC   pMM900 from pKO-1 & pNOP42
RC   pMM727 from pKO-1 & pEW2762
RC   pMM701 from pKO-1 & pMM1
RA   Wong E.M., Muesing M.A., Polisky B.;
RT   "Temperature-sensitive copy number mutants of CoIE1 are located in
RT   an untranslated region of the plasmid genome";
RL   Proc. Natl. Acad. Sci. U.S.A. 79:3570-3574(1982).
CC   An expression vector with temperature-dependent copy number
CC   amplification and lacIq control of insert. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pEW27)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli D1205)(E.coli)(E.coli JM101)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. ColE1 6646bp
FT                   2. E. coli, lac promoter
FT                   -> pBGP120 17299bp [EcoRI]
FT                   1. pBGP120 17299bp
FT                   mutation, to temperature sensitive copy number
FT                   -> pEW27 17299bp [EcoRI]"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-EcoRI-HindIII-SstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)
FT                   CdE1"
FT   CDS             0..0
FT                   /note="ORI E. coli cop(ts) gene"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;