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ID   PEX34B     preliminary; circular DNA; SYN; 3200 BP.
AC   M18855;
DT   15-MAR-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   Schistosoma plasmid vector pEx34b - incomplete, MS2 polyme.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-100
RC   pEx34b from pPLc24 & pEx31
RA   Klinkert M.Q., Ruppel A., Felleisen R., Link G., Beck W.D.;
RT   "Expression of diagnostic 31/32 kilodalton proteins of Schistosoma
RT   mansoni as fusions with bacteriophage MS2 polymerase";
RL   Mol. Biochem. Parasitol. 27:233-240(1988).
RN   [2]
RC   plasmid from pPLc24 & oligo
RC   pEx30 from plasmid & pBR322
RC   pSV010 from pSV01
RC   pEx31 from plasmid & pSV010
RC   pEVP1 from pPLc24 & pFMDV88, FMDV
RC   pE52 from pFMDV88, FMDV
RC   pE34 from pE52
RC   pEx31b from pEx31
RC   pEProt* from pEx31b & pFMDV100, FMDV
RC   pE12, pE12VPg, pEVPg, pE20 from pEProt*
RC   pE56 from pPLc24 & pFMDV100, FMDV
RC   pE16 from pEx31b & FMDV
RA   Strebel K., Beck E., Strohmeier K., Schaller H.;
RT   "Characterization of foot and mouth disease virus gene products
RT   with antisera against bacterially synthesized fusion proteins";
RL   J. Virol. 57:983-991(1986).
CC   NCBI gi: 208356
CC   NM (pEx34b)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA (bacteriophage MS2)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. lambda, pL
FT                   2. MS2 polymerase gene
FT                   -> pPLc24 3143bp [BamHI, HindIII near MS2]
FT                   1. pPLc24 EcoRI 3143bp,
FT                   \ between lambda promoter and MS2
FT                   fill in
FT                   BamHI 3143bp, near MS2
FT                   S1 nuclease
FT                   EcoRI linker 6bp gaattc
FT                   -> plasmid 3140bp
FT                   1. pSV01
FT                   -> pSV010
FT                   1. plasmid remove small EcoRI-HindIII, 3000bp
FT                   2. pSV010 EcoRI-BamHI-PstI-BglII-HindIII, MCS
FT                   -> pEx31 3000bp
FT                   1. pPLc24 3143bp
FT                   2. pEx31 50bp
FT                   -> pEx34b 3200bp"
FT   misc_binding    0..0
FT                   /note="MCS EcoRI-BamHI-PstI-BglII-XbaI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI Schistosoma"
FT   misc_feature    16..17
FT                   /note="SIT cDNA insertion site"
FT   CDS             0..0
FT                   /note="ANT E. coli amp gene"
FT   promoter        0..0
FT                   /note="PRO lambda pL"
FT   CDS             0..0
FT                   /note="GEN MS2 polymerase gene"
SQ   Sequence 100 BP; 23 A; 21 C; 32 G; 24 T; 0 other;
     aaatccttgt catgggaatt cgcgcgaccg gatccgggca acgttgttgc cattgctgca
     ggcggagaac tggtaggtat ggaagatctc tagaagcttg