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ID   PEYDG1     preliminary; circular DNA; SYN; 8600 BP.
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AC   ATCC37316;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pEYDG1 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pDG4delta15 from pBR322 & Tn5
RC   pEYDG1 from pTJS37 & pDG4delta15
RA   Yakobson E.A., Guiney D.G.;
RT   "Conjugal transfer of bacterial chromosomes mediated by the RK2
RT   plasmid transfer origin cloned into transposon Tn5";
RL   J. Bacteriol. 160:451-453(1984).
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RN   [2]
RC   pTJS37 from RK2, oriT
RC   [pTJS82 from RK2, oriT]
RA   Schmidhauser T.J.;
RT   ;
RL   Unpublished (1984).
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CC   Plasmid for use in RK2-oriT-mediated conjugal transfer of gram
CC   negative bacterial chromosomes.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pEYDG1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli HB101)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove HaeII-HaeII,
FT                   \ HaeII = 237 418 439 499 553 780 1210 1649 1732
FT                   -> pDG4delta15 [amp, oriV, no tet]
FT                   1. pDG4delta15
FT                   transposition
FT                   2. Tn5 5818bp, kan gene
FT                   -> plasmid [kan, no tet]
FT                   1. RK2 BamHI-BamHI 760bp, oriT
FT                   -> pTJS82
FT                   1. pTJS82 BamHI-BamHI 760bp, oriT
FT                   2. plasmid BamHI
FT                   -> pEYDG1 8600bp [kan]"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_feature    0..0
FT                   /note="IS50R"
FT   rep_origin      0..0
FT                   /note="ORI E. coli RK2 oriT"
FT   misc_binding    0..0
FT                   /note="SIT unique SmaI"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
FT   misc_feature    0..0
FT                   /note="IS50L"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
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