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ID   PF3        preliminary; circular DNA; SYN; 252 BP.
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AC   X05601;
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DT   29-NOV-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Saccharomyces/Kluyveromyces plasmid vector pF3 - incomplete, LEU2.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-252
RC   pGKL2-LEU2 from pGKL2 & pAJ50
RC   pF3 from pGKL2 & pAJ50
RC   pFJ04 from pF3 & pUC13
RC   pFJ07 from pFJ04 & YIp5
RC   YIp5/ARS1 from YIp5 & YRp7
RA   Fujimura H., Hishinuma F., Gunge N.;
RT   "Terminal segment of Kluyveromyces lactis linear DNA plasmid pGKl2
RT   supports autonomous replication of hybrid plasmids in Saccharomyces
RT   cerevisiae";
RL   Curr. Genet. 12:99-104(1987).
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RN   [2]
RC   pAJ50 from pBR322 & yeast 2 micron & LEU2 gene & Tn903
RA   Davies J.;
RT   ;
RL   Unpublished (1987).
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RN   [3]
RC   pKR1BU3 from pBR322 & K. lactis URA3 gene & K. lactis ARS & Tn903
RA   Dickson R.;
RT   ;
RL   Unpublished (1987).
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CC   NCBI gi: 58123
CC   NM (pF3)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(Saccharomyces cerevisiae)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (Kluyveromyces lactis pGKL2)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   transposition
FT                   2. Tn903 3094bp, kan gene
FT                   -> plasmid 7455bp
FT                   1. plasmid 7455bp
FT                   2. yeast 2 micron 2200bp, ori
FT                   -> plasmid2 9700bp
FT                   1. plasmid2 PstI 9700bp, amp gene
FT                   2. yeast PstI-PstI 2700bp, LEU2 gene
FT                   -> pAJ50 12400bp
FT                   1. pGKL2 637bp
FT                   2. pAJ50 PstI-PstI 2700bp, LEU2 gene
FT                   -> pF3 3300bp"
FT   misc_feature    1..121
FT                   /note="from Kluyveromyces lactis pGKL2"
FT   CDS             122..252
FT                   /note="ANT yeast LEU2 gene; partial"
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SQ   Sequence 252 BP; 67 A; 52 C; 27 G; 106 T; 0 other;
     tcgagatatg acctaaacat aaaaaaacac gtaaaacata ctttgggttg cttttttttg
     acctgatttc aacttttatt agcttaaaaa cccaacttta ttttttatat atataccttt
     tttctgttac acctaacttt ttgtgtggtg ccctcctcct tgtcaatatt aatgttaaag
     tgcaattctt tttccttatc acgttgagcc attagtatca atttgcttac ctgtattcct
     ttacatcctc ct
//