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ID   PFASTBAC1  preliminary; circular DNA; SYN; 4775 BP.
AC   IG9001;
DT   01-FEB-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pFastBac1 - incomplete.
KW   cloning vector; baculovirus.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pSFV1 from pBR322 & SFV
RA   Luckow V.A., Lee S.C., Barry G.F., Olins P.O.;
RT   "Efficient generation of infectious recombinant baculoviruses by
RT   site-specific transposon-mediated insertion of foreign genes into
RT   a baculovirus genome propagated in Escherichia coli";
RL   J. Virol. 67:4566-4579(1993).
CC   NM (pFastBac1)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (baculovirus)
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. pUC19 2686bp
FT                   2. E. coli 2000bp, gent gene
FT                   -> pFastBac1 4775bp"
FT   rep_origin      300..400
FT                   /note="ORI phage f1"
FT   misc_binding    127..127
FT                   /note="SIT NgoAIV"
FT   CDS             2710..3750
FT                   /note="GEN E. coli amp gene"
FT   misc_binding    835..835
FT                   /note="SIT BamHI"
FT   misc_binding    1004..1004
FT                   /note="SIT PvuI"
FT   misc_binding    1151..1151
FT                   /note="SIT FspI"
FT   misc_binding    1266..1266
FT                   /note="SIT GsuI"
FT   rep_origin      1500..2000
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    1850..1850
FT                   /note="SIT AlwNI"
FT   misc_binding    2366..2366
FT                   /note="SIT SapI"
FT   misc_feature    2500..2500
FT                   /note="phage Tn7 right"
FT   misc_binding    2707..2707
FT                   /note="SIT MscI"
FT   misc_binding    2710..2710
FT                   /note="SIT BstXI"
FT   CDS             2710..3750
FT                   /note="GEN gent gene"
FT   misc_binding    2765..2765
FT                   /note="SIT SstII"
FT   misc_binding    2823..2823
FT                   /note="SIT EcoRV"
FT   misc_binding    3176..3176
FT                   /note="SIT Esp3I"
FT   misc_binding    3225..3225
FT                   /note="SIT Tth111I"
FT   misc_binding    3410..3410
FT                   /note="SIT Bsp1407I"
FT   misc_binding    3750..3750
FT                   /note="SIT BbsI"
FT   misc_binding    3879..3879
FT                   /note="SIT SnaBI"
FT   misc_binding    3882..3882
FT                   /note="SIT Bst1107I"
FT   promoter        3900..4030
FT                   /note="PRO Polh"
FT   misc_binding    4015..4015
FT                   /note="SIT BamFI"
FT   misc_binding    4032..4137
FT                   /note="MCS BamHI-RsrII-BssHII-EcoRI-StuI-SalI-SstI-
FT                   SpeI-NotI-NspV-XbaI-PstI-XhoI-SphI-KpnI-HindIII"
FT   terminator      4200..4400
FT                   /note="TER SV40 polyA"
FT   misc_binding    4252..4252
FT                   /note="SIT MunI"
FT   misc_binding    4263..4263
FT                   /note="SIT HpaI"
FT   misc_binding    4401..4401
FT                   /note="SIT BclI"
FT   misc_binding    4416..4416
FT                   /note="SIT AvrII"
FT   misc_feature    4416..4416
FT                   /note="phage Tn7 left"
FT   CDS             4500..4775
FT                   /note="GEN E. coli lacZ gene"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;