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ID   PFC54T     preliminary; circular DNA; SYN; 5610 BP.
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AC   ATCC39789;
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DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pFC54.t - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pFC54 from pBR322 & lambda, pL
RC   pHCW801 from IL-2 gene & positive retroregulatory element
RC   pFC54.t from pFC54 & pHCW801
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,677,063 dated June 30, 1987.
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RN   [2]
RC   pFC54 from pBR322 & lambda, pL
RC   pHCW801 from IL-2 gene & positive retroregulatory element
RC   pFC54.t from pFC54 & pHCW801
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,677,064 dated June 30, 1987.
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RN   [3]
RC   pFC54 from pBR322 & lambda, pL
RC   pHCW801 from IL-2 gene & positive retroregulatory element
RC   pFC54.t from pFC54 & pHCW801
RA   ;
RT   ;
RL   Unpublished (1987).
RL   U.S. Patent No. 4,666,848 dated May 19, 1987.
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CC   The clone contains a lambda pL promoter. [3]
CC   This was constructed from a SphI/XbaI fragment of pFC54 ligated to a
CC   655 bp SphI/XbaI fragment of pHCW801 containing C-terminal 255 bp of
CC   Ser125 IL-2, 36 bp of 3' untranslated IL-2 cDNA and 394 bp of the
CC   positive retroregulatory element. [3]
CC   Construct contains a positive retroregulatory element which increases
CC   the level of production of the IL2 (Ser125) using a temperature
CC   regulated control cassette in a temperature-regulated ColE1 copy
CC   mutant plasmid vector. (personal communication)
CC   This should not be grown at temperatures exceeding 30C. The depositor
CC   recommends using ampicillin at 100 ug/ml. The strain is temperature
CC   inducible. (personal communication)
CC   Medium is 1315 1065 plus ampicillin (100 ug/ul).
CC   NM (pFC54.t)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   2. lambda, pL
FT                   -> pFC54
FT                   1. IL-2 gene/IL-2 positive retroregulatory element
FT                   -> pHCW801
FT                   1. pFC54 remove small SphI-XbaI, 5000bp
FT                   2. pHCW801 SphI/XbaI 655bp, IL-2
FT                   -> pFC54.t 5610bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI-HindIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda PL"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
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SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;
     n
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