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ID   PFCE4      preliminary; circular DNA; SYN; 60 BP.
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AC   IG8022;
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DT   01-DEC-1994 (Rel. 10, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pFCE4 - incomplete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pFCE4+, pFCE4- from pOTS & pD4, phage f1 ori
RA   Lorenzetti R., Dani M., Lappi D.A., Martineau D., Casati M.,
RA   Monaco L., Shatzman A., Rosenberg M., Soria M.;
RT   "Plasmid pFCE4: a new system of Escherichia coli
RT   expression-modification vectors";
RL   Gene 39:85-87(1985).
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CC   NM (pFCE4)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pOTS EcoRI 7400bp
FT                   2. pD4 EcoRI-? 1260bp 4292..5552, phage f1 ori
FT                   -> pFCE4+ 8700bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 60 BP; 16 A; 14 C; 14 G; 16 T; 0 other;
     aattgttatc taaggaaata cttacatatg gatcctctac gccggacgca tcgtggccgg
//