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ID   PFDA2      preliminary; circular DNA; SYN; 1630 BP.
AC   ATCC37375;
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pfdA2 - incomplete.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   lambda NM616 from lambda plac5
RC   lambda CH616 from lambda NM616 & pTM12, phage fd gene 2
RC   pfdA2 from pfdA1 & linker
RC   pfdA3 from pfdA1 & pSV2-cat
RC   pfdA2E from pfdA2
RC   pfdA4 from pfdA2E & pHM2
RC   [pBA from pBR series]
RC   pfdA8 from pfdA2 & pUR222 & pBA
RC   pfdB2 from pfdA2 & pIG414-RB
RA   Geider K., Hohmeyer C., Haas R., Meyer T.F.;
RT   "A plasmid cloning system utilizing replication and packaging
RT   functions of the filamentous bacteriophage fd";
RL   Gene 33:341-349(1985).
RN   [2]
RC   pTM11 from pBR325 & phage fd 11, gene 2
RC   pTM12 from pTM11
RC   pfdA1 from phage fd ori & kan gene
RC   pfdCO from pfdA1 & pTM12
RA   Meyer T.F., Geider K.;
RT   "Cloning of bacteriophage fd gene 2 and construction of a plasmid
RT   dependent on fd gene 2 protein";
RL   Proc. Natl. Acad. Sci. U.S.A. 78:5416-5420(1981).
RN   [3]
RC   pHM2 from pBR322 & Tn9
RA   Matzura H.;
RT   ;
RL   Unpublished (1984).
RN   [4]
RC   pIG414-RB from pBR322 & phage fd ori
RA   Sommer R., Herrman R., Schaller H.;
RT   ;
RL   Unpublished (1984).
CC   The plasmid is lost at 42C if grown without antibiotic.  The host
CC   E. coli strain must express gene 2 of filamentous bacteriophages.
CC   Vector with bacteriophage fd replication system.
CC   Medium is 1236 LB plus kanamycin.
CC   NM (pfdA2)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   HO (E.coli BHB2600)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. phage fd HaeII 6408bp 5572..5572
FT                   2. E. coli, kan gene/cat gene
FT                   -> phage fd 106
FT                   1. phage fd remove 579bp 5830..6408..1,
FT                   \ between gene2 and ori/5830bp
FT                   EcoRI linker 6bp gaattc
FT                   -> phage fd 11 5830bp
FT                   1. phage fd 11 BamHI-EcoRI 184bp 5646..5830, ori
FT                   2. phage fd 106 BamHI-EcoRI 2200bp, kan gene/3' cat
FT                   \ cat EcoRI to fd BamHI 5646/5572..-..5646
FT                   -> pfdA1 2400bp
FT                   1. pfdA1 HaeII-HaeII
FT                   \ fd HaeII = 2715 4748 5565 5573
FT                   DNA polymerase I:DNA polymerase I
FT                   EcoRI linker 6bp gaattc:EcoRI linker 6bp gaattc
FT                   EcoRI-EcoRI
FT                   -> pfdA2o
FT                   1. pfdA2o remove HaeII-HaeII, 1630bp
FT                   -> pfdA2 1630bp"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI-HaeII"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage fd"
FT   CDS             0..0
FT                   /note="ANT E. coli kanamycin resistance gene (kan)"
SQ   Sequence 1 BP; 0 A; 0 C; 0 G; 0 T; 1 other;