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ID   PFE373     preliminary; circular DNA; SYN; 3900 BP.
AC   K01478;
DT   07-NOV-1984 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pFE373 - incomplete, methotroxate-resist DHFR.
KW   cloning vector; dihydrofolate reductase; DHFR; methotrexate-resistant.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RC   pFE504, pFE506 from pBR322 & ColE1::Tn7, R483 Tn7 DHFR I gene
RC   pFE332 from pBR322 & R67, DHFR II gene
RC   pFE364 from pFE332
RC   pFE413 from pSC101 & R67, DHFR II gene
RC   pFE420 from pFE413
RC   pFE425 from pFE413
RC   pFE373 from pBR322 & R388, DHFR II gene
RA   Fling M.E., Elwell L.P.;
RT   "Protein expression in Escherichia coli minicells containing
RT   recombinant plasmids specifying trimethoprim-resistant
RT   dihydrofolate reductases";
RL   J. Bacteriol. 141:779-785(1980).
CC   A recombinant plasmid expressed inserted DNA sequences coding for
CC   prokaryotic methotrexate-resistant dihydrofolate reductase (DHFR)
CC   of unspecified origin, using a SV40 early promoter region and
CC   polyadenylation signals from the rabbit globin gene. The DHFR gene
CC   insert begins at base 4. The plasmids were grown in mouse
CC   fibroblasts and produced chimeric mRNA coding for the prokaryotic
CC   enzyme. The mouse fibroblasts became Mtx resistant.
CC   [1] also reports a DHFR sequence found in plasmid pFE364.
CC   NCBI gi: 208996
CC   NM (pFE373)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(mouse fibroblast cells)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR (pFE364)
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. E. coli, DHFR II gene
FT                   -> R388
FT                   1. pBR322 BamHI 4361bp 376..376
FT                   2. SV40 HindIII-PvuII 344bp 5172..5243..273,
FT                   \ early promoter
FT                   3. R388 BamHI-BamHI 1300bp, DHFR II gene
FT                   4. rabbit, beta-globin gene polyA
FT                   -> pFE373 3900bp"
FT   promoter        0..0
FT                   /note="PRO SV40 early gene"
FT   polyA_signal    0..0
FT                   /note="PLA rabbit beta-globin gene"
FT   CDS             43..>90
FT                   /note="GEN E. coli dihydrofolate reductase gene
FT                   (DHFR); NCBI gi: 208997"
SQ   Sequence 90 BP; 24 A; 19 C; 25 G; 22 T; 0 other;
     tttgcttgtg tcgccataat agattcacaa gaaggattcg acatgggtaa aagtagcggc
     gaagccaacg ctcccgttgc agggcagttt