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ID   PFH118     preliminary; circular DNA; SYN; 120 BP.
AC   K00516;
DT   12-MAR-1984 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
DE   E. coli plasmid vector pFH118 - incomplete, RNA-I mutant coding reg.
KW   cloning vector.
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
RN   [1]
RP   1-120
RC   pFH118 from RSF1050
RC   pDM246, pDM247, pDM248 from RSF1050 & pFH118
RC   pDM251, pDM252, pDM253, pDM254 from RSF1050 & pACYC184
RA   Moser D.R., Campbell J.L.;
RT   "Characterization and complementation of pMB1 copy number mutant:
RT   effect of RNA I gene dosage on plasmid copy number and
RT   incompatibility";
RL   J. Bacteriol. 154:809-818(1983).
CC   See #RSF1050 for the normal RNA I sequence.
CC   NCBI gi: 208998
CC   NM (pFH118)
CC   CM (no)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
FH   Key             Location/Qualifiers
FT   misc_feature    0..0
FT                   /note="1. RSF1050 EcoRI 10300bp,
FT                   \ 520 bp 5' to ori in RNAI CDS
FT                   EcoRI linker 6bp gaattc
FT                   mutagenesis
FT                   -> pFH118 10300bp"
FT   misc_feature    79..94
FT                   /note="insertion in RNA I coding region;
FT                   making high copy number"
SQ   Sequence 120 BP; 31 A; 26 C; 30 G; 33 T; 0 other;
     agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     ttgatccggc aaacaaacgg aattccaaac aaaccaccgc tggtagcggt ggtttttttg